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7dzu
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7dzu is ON HOLD until Paper Publication Authors: Sagong, H.-Y., Kim, K.-J. Description: Cyrstal structure of PETase K169A mutant from Rhizobacter g...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Cyrstal structure of PETase K169A mutant from Rhizobacter gummiphilus== | |
| + | <StructureSection load='7dzu' size='340' side='right'caption='[[7dzu]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7dzu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobacter_gummiphilus Rhizobacter gummiphilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DZU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dzu OCA], [https://pdbe.org/7dzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dzu RCSB], [https://www.ebi.ac.uk/pdbsum/7dzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dzu ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1W6L588_9BURK A0A1W6L588_9BURK] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The development of a superb polyethylene terephthalate (PET) hydrolyzing enzyme requires an accurate understanding of the PET decomposition mechanism. However, studies on PET degrading enzymes, including the PET hydrolase from Ideonella sakaiensis (IsPETase), have not provided sufficient knowledge of the molecular mechanisms for the hardly accessible substrate. Here, we report a novel PET hydrolase from Rhizobacter gummiphilus (RgPETase), which has a hydrolyzing activity similar to IsPETase toward microcrystalline PET but distinct behavior toward low crystallinity PET film. Structural analysis of RgPETase reveals that the enzyme shares the key structural features of IsPETase for high PET hydrolysis activity but has distinguished structures at the surface-exposed regions. RgPETase shows a unique conformation of the wobbling tryptophan containing loop (WW-loop) and change of the electrostatic surface charge on the loop dramatically affects the PET-degrading activity. We further show that effect of the electrostatic surface charge to the activity varies depending on locations. This work provides valuable information underlying the uncovered PET decomposition mechanism. | ||
| - | + | Implications for the PET decomposition mechanism through similarity and dissimilarity between PETases from Rhizobacter gummiphilus and Ideonella sakaiensis.,Sagong HY, Son HF, Seo H, Hong H, Lee D, Kim KJ J Hazard Mater. 2021 Aug 15;416:126075. doi: 10.1016/j.jhazmat.2021.126075. Epub , 2021 May 11. PMID:34492896<ref>PMID:34492896</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Kim | + | <div class="pdbe-citations 7dzu" style="background-color:#fffaf0;"></div> |
| - | [[Category: Sagong | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Rhizobacter gummiphilus]] | ||
| + | [[Category: Kim K-J]] | ||
| + | [[Category: Sagong H-Y]] | ||
Current revision
Cyrstal structure of PETase K169A mutant from Rhizobacter gummiphilus
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