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7wag

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Crystal structure of MurJ squeezed form

Structural highlights

7wag is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.55Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MURJ_ECOLI Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.[HAMAP-Rule:MF_02078]^[1] ^[2] ^[3]

Publication Abstract from PubMed

The bacterial peptidoglycan enclosing the cytoplasmic membrane is a fundamental cellular architecture. The integral membrane protein MurJ plays an essential role in flipping the cell wall building block Lipid II across the cytoplasmic membrane for peptidoglycan biosynthesis. Previously reported crystal structures of MurJ have elucidated its V-shaped inward- or outward-facing forms with an internal cavity for substrate binding. MurJ transports Lipid II using its cavity through conformational transitions between these two forms. Here, we report two crystal structures of inward-facing forms from Arsenophonus endosymbiont MurJ and an unprecedented crystal structure of Escherichia coli MurJ in a "squeezed" form, which lacks a cavity to accommodate the substrate, mainly because of the increased proximity of transmembrane helices 2 and 8. Subsequent molecular dynamics simulations supported the hypothesis that the squeezed form is an intermediate conformation. This study fills a gap in our understanding of the Lipid II flipping mechanism.

Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms.,Kohga H, Mori T, Tanaka Y, Yoshikaie K, Taniguchi K, Fujimoto K, Fritz L, Schneider T, Tsukazaki T Structure. 2022 May 23. pii: S0969-2126(22)00182-4. doi:, 10.1016/j.str.2022.05.008. PMID:35660157^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Inoue A, Murata Y, Takahashi H, Tsuji N, Fujisaki S, Kato J. Involvement of an essential gene, mviN, in murein synthesis in Escherichia coli. J Bacteriol. 2008 Nov;190(21):7298-301. doi: 10.1128/JB.00551-08. Epub 2008 Aug, 15. PMID:18708495 doi:http://dx.doi.org/10.1128/JB.00551-08
↑ Ruiz N. Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coli. Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15553-7. doi:, 10.1073/pnas.0808352105. Epub 2008 Oct 1. PMID:18832143 doi:http://dx.doi.org/10.1073/pnas.0808352105
↑ Sham LT, Butler EK, Lebar MD, Kahne D, Bernhardt TG, Ruiz N. Bacterial cell wall. MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis. Science. 2014 Jul 11;345(6193):220-2. doi: 10.1126/science.1254522. PMID:25013077 doi:http://dx.doi.org/10.1126/science.1254522
↑ Kohga H, Mori T, Tanaka Y, Yoshikaie K, Taniguchi K, Fujimoto K, Fritz L, Schneider T, Tsukazaki T. Crystal structure of the lipid flippase MurJ in a "squeezed" form distinct from its inward- and outward-facing forms. Structure. 2022 May 23. pii: S0969-2126(22)00182-4. doi:, 10.1016/j.str.2022.05.008. PMID:35660157 doi:http://dx.doi.org/10.1016/j.str.2022.05.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7wag"

@@ Line 3: / Line 3: @@
 <StructureSection load='7wag' size='340' side='right'caption='[[7wag]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7wag]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7wag]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAG FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wag OCA], [https://pdbe.org/7wag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wag RCSB], [https://www.ebi.ac.uk/pdbsum/7wag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wag ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/MURJ_ECOLI MURJ_ECOLI]] Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.[HAMAP-Rule:MF_02078]<ref>PMID:18708495</ref> <ref>PMID:18832143</ref> <ref>PMID:25013077</ref>
+[https://www.uniprot.org/uniprot/MURJ_ECOLI MURJ_ECOLI] Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.[HAMAP-Rule:MF_02078]<ref>PMID:18708495</ref> <ref>PMID:18832143</ref> <ref>PMID:25013077</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Fujimoto, K]]
+[[Category: Fujimoto K]]
-[[Category: Kohga, H]]
+[[Category: Kohga H]]
-[[Category: Tanaka, Y]]
+[[Category: Tanaka Y]]
-[[Category: Taniguchi, K]]
+[[Category: Taniguchi K]]
-[[Category: Tsukazaki, T]]
+[[Category: Tsukazaki T]]
-[[Category: Yoshikaie, K]]
+[[Category: Yoshikaie K]]
-[[Category: 14 transmembrane helice]]
-[[Category: Inner membrane]]
-[[Category: Lipid ii]]
-[[Category: Lipid transport]]
-[[Category: Mop superfamily]]

7wag

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Current revision

Crystal structure of MurJ squeezed form

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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