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7x17
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Pseudomonas NRPS protein, AmbB-TC bound to Ppant-L-Ala== | |
| - | + | <StructureSection load='7x17' size='340' side='right'caption='[[7x17]], [[Resolution|resolution]] 2.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[7x17]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7X17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7X17 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | |
| - | [[Category: | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=868:S-[2-[3-[[(2S)-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl]+(2R)-2-azanylpropanethioate'>868</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7x17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7x17 OCA], [https://pdbe.org/7x17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7x17 RCSB], [https://www.ebi.ac.uk/pdbsum/7x17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7x17 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/AMBB_PSEAE AMBB_PSEAE] Involved in the biosynthesis of the antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid (AMB), a non-proteinogenic amino acid which is toxic for prokaryotes and eukaryotes (PubMed:20543073, PubMed:25814981). Adenylates L-alanine and loads it onto its peptidyl carrier domain via a thioester linkage to the phosphopanthetheine moiety. In addition, loads activated L-Ala in trans onto the second carrier domain of AmbE (PubMed:25814981). Can also activate L-Ser, Gly and D-Ala, albeit to a lower extent (PubMed:25814981). The condensation domain of AmbB probably condenses the activated L-Ala and the L-Glu loaded on AmbE to form a L-Glu-L-Ala dipeptide at the first carrier domain of AmbE (PubMed:25814981).<ref>PMID:20543073</ref> <ref>PMID:25814981</ref> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
| + | [[Category: Bharath SR]] | ||
| + | [[Category: ChuYuanKee M]] | ||
| + | [[Category: Song H]] | ||
Current revision
Structure of Pseudomonas NRPS protein, AmbB-TC bound to Ppant-L-Ala
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