1gn7

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(New page: 200px<br /><applet load="1gn7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gn7" /> '''NMR STRUCTURE OF AN INTRAMOLECULAR DNA TRIPL...)
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[[Image:1gn7.gif|left|200px]]<br /><applet load="1gn7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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'''NMR STRUCTURE OF AN INTRAMOLECULAR DNA TRIPLEX CONTAINING AN N7-GLYCOSYLATED GUANINE, 8 STRUCTURES'''<br />
 
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==Overview==
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==NMR STRUCTURE OF AN INTRAMOLECULAR DNA TRIPLEX CONTAINING AN N7-GLYCOSYLATED GUANINE, 8 STRUCTURES==
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The three-dimensional structure of a pyrimidine-purine-pyrimidine DNA, triplex containing an N7-glycosylated guanine (7G) in the third strand has, been determined by NMR spectroscopy, restrained molecular dynamics, calculations, and complete relaxation matrix refinement. Glycosylation of, the guanine at the N7 position permits it to adopt a conformation such, that the Hoogsteen face of the base mimics the arrangement of hydrogen, bond donors seen in protonated cytosine. The NMR data confirm the, previously proposed hydrogen bonding scheme of the 7G x G x C triplet. The, three-dimensional structure of the triplex accommodates the 7G with less, distortion of the phosphodiester backbone than would be required for an, N9-glycosylated guanine in the same sequence position, but some changes in, the positions of the phosphodiester backbone are present compared to a C+, x G x C triplet. The structure provides a rationale for the observations, that 7G binds to Watson-Crick G x C base pairs with higher specificity and, affinity than guanine, but with a lower stability at pH 5.2 than would be, provided by a canonical C+ x G x C triplet.
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<StructureSection load='1gn7' size='340' side='right'caption='[[1gn7]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gn7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GN7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GN7:N7-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>GN7</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gn7 OCA], [https://pdbe.org/1gn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gn7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gn7 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structure of a pyrimidine-purine-pyrimidine DNA triplex containing an N7-glycosylated guanine (7G) in the third strand has been determined by NMR spectroscopy, restrained molecular dynamics calculations, and complete relaxation matrix refinement. Glycosylation of the guanine at the N7 position permits it to adopt a conformation such that the Hoogsteen face of the base mimics the arrangement of hydrogen bond donors seen in protonated cytosine. The NMR data confirm the previously proposed hydrogen bonding scheme of the 7G x G x C triplet. The three-dimensional structure of the triplex accommodates the 7G with less distortion of the phosphodiester backbone than would be required for an N9-glycosylated guanine in the same sequence position, but some changes in the positions of the phosphodiester backbone are present compared to a C+ x G x C triplet. The structure provides a rationale for the observations that 7G binds to Watson-Crick G x C base pairs with higher specificity and affinity than guanine, but with a lower stability at pH 5.2 than would be provided by a canonical C+ x G x C triplet.
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==About this Structure==
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Solution structure of an intramolecular DNA triplex containing an N7-glycosylated guanine which mimics a protonated cytosine.,Koshlap KM, Schultze P, Brunar H, Dervan PB, Feigon J Biochemistry. 1997 Mar 4;36(9):2659-68. PMID:9054573<ref>PMID:9054573</ref>
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1GN7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GN7 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of an intramolecular DNA triplex containing an N7-glycosylated guanine which mimics a protonated cytosine., Koshlap KM, Schultze P, Brunar H, Dervan PB, Feigon J, Biochemistry. 1997 Mar 4;36(9):2659-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9054573 9054573]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1gn7" style="background-color:#fffaf0;"></div>
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[[Category: Feigon, J.]]
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== References ==
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[[Category: Koshlap, K.M.]]
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<references/>
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[[Category: Schultze, P.]]
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__TOC__
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[[Category: deoxyribonucleic acid]]
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</StructureSection>
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[[Category: n7-guanine]]
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[[Category: Large Structures]]
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[[Category: triplex]]
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[[Category: Feigon J]]
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[[Category: Koshlap KM]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:34:24 2007''
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[[Category: Schultze P]]

Current revision

NMR STRUCTURE OF AN INTRAMOLECULAR DNA TRIPLEX CONTAINING AN N7-GLYCOSYLATED GUANINE, 8 STRUCTURES

PDB ID 1gn7

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