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1k5f

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[[Image:1k5f.png|left|200px]]
 
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{{STRUCTURE_1k5f| PDB=1k5f | SCENE= }}
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==SOLUTION STRUCTURE OF THE S-STYRENE ADDUCT IN THE RAS61 SEQUENCE==
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<StructureSection load='1k5f' size='340' side='right'caption='[[1k5f]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k5f]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K5F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABS:(S)-(N-PHENYL-2-HYDROXY-ETHYL)-2-DEOXY-ADENOSINE-5-MONOPHOSPHATE'>ABS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k5f OCA], [https://pdbe.org/1k5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k5f RCSB], [https://www.ebi.ac.uk/pdbsum/1k5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k5f ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Conformations of (R)-beta-(N(6)-adenyl)styrene oxide and (S)-beta-(N(6)-adenyl)styrene oxide adducts at position X(6) in d(CGGACXAGAAG).d(CTTCTTGTCCG), incorporating codons 60, 61 (underlined), and 62 of the human N-ras protooncogene, were refined from (1)H NMR data. These were designated as the beta-R(61,2) and beta-S(61,2) adducts. A total of 533 distance restraints and 162 dihedral restraints were used for the molecular dynamics calculations of the beta-S(61,2) adduct, while 518 distances and 163 dihedrals were used for the beta-R(61,2) adduct. The increased tether length of the beta-adducts results in two significant changes in adduct structure as compared to the corresponding alpha-styrenyl adducts [Stone, M. P., and Feng, B. (1996) Magn. Reson. Chem. 34, S105-S114]. First, it reduces the distortion introduced into the DNA duplex. For both the beta-R(61,2) and beta-S(61,2) adducts, the styrenyl moiety was positioned in the major groove of the duplex with little steric hindrance. Second, it mutes the influence of stereochemistry at the alpha-carbon such that both the beta-R(61,2) and beta-S(61,2) adducts exhibit similar conformations. The results were correlated with site-specific mutagenesis experiments that revealed the beta-R(61,2) and beta-S(61,2) adducts were not mutagenic and did not block polymerase bypass.
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===SOLUTION STRUCTURE OF THE S-STYRENE ADDUCT IN THE RAS61 SEQUENCE===
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The nonmutagenic (R)- and (S)-beta-(N(6)-adenyl)styrene oxide adducts are oriented in the major groove and show little perturbation to DNA structure.,Hennard C, Finneman J, Harris CM, Harris TM, Stone MP Biochemistry. 2001 Aug 21;40(33):9780-91. PMID:11502171<ref>PMID:11502171</ref>
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{{ABSTRACT_PUBMED_11502171}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1k5f" style="background-color:#fffaf0;"></div>
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[[1k5f]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5F OCA].
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== References ==
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[[Category: Finneman, J.]]
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<references/>
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[[Category: Harris, C M.]]
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__TOC__
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[[Category: Harris, T M.]]
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</StructureSection>
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[[Category: Hennard, C.]]
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[[Category: Large Structures]]
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[[Category: Stone, M P.]]
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[[Category: Finneman J]]
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[[Category: Dna]]
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[[Category: Harris CM]]
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[[Category: Dna adduct]]
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[[Category: Harris TM]]
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[[Category: Solution structure of dna]]
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[[Category: Hennard C]]
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[[Category: Styrene]]
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[[Category: Stone MP]]

Current revision

SOLUTION STRUCTURE OF THE S-STYRENE ADDUCT IN THE RAS61 SEQUENCE

PDB ID 1k5f

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