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1lb7

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[[Image:1lb7.jpg|left|200px]]
 
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==IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1==
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The line below this paragraph, containing "STRUCTURE_1lb7", creates the "Structure Box" on the page.
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<StructureSection load='1lb7' size='340' side='right'caption='[[1lb7]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lb7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LB7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lb7 OCA], [https://pdbe.org/1lb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lb7 RCSB], [https://www.ebi.ac.uk/pdbsum/1lb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lb7 ProSAT]</span></td></tr>
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{{STRUCTURE_1lb7| PDB=1lb7 | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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'''IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1'''
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== Publication Abstract from PubMed ==
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==Overview==
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A panel of 22 naive peptide libraries was constructed in a polyvalent phage display format and sorted against insulin-like growth factor-1 (IGF-1). The libraries were pooled to achieve a total diversity of 4.4 x 10(11). After three rounds of selection, the majority of the phage clones bound specifically to IGF-1, with a disulfide-constrained CX(9)C scaffold dominating the selection. Four monovalently displayed sub-libraries were designed on the basis of these conserved motifs. Sub-library maturation in a monovalent format yielded an antagonistic peptide that inhibited the interactions between IGF-1 and two cell-surface receptors and those between IGF-1 and two soluble IGF binding proteins with micromolar potency. NMR analysis revealed that the peptide is highly structured in the absence of IGF-1, and peptides that preorganize the binding elements were selected during the sorting.
A panel of 22 naive peptide libraries was constructed in a polyvalent phage display format and sorted against insulin-like growth factor-1 (IGF-1). The libraries were pooled to achieve a total diversity of 4.4 x 10(11). After three rounds of selection, the majority of the phage clones bound specifically to IGF-1, with a disulfide-constrained CX(9)C scaffold dominating the selection. Four monovalently displayed sub-libraries were designed on the basis of these conserved motifs. Sub-library maturation in a monovalent format yielded an antagonistic peptide that inhibited the interactions between IGF-1 and two cell-surface receptors and those between IGF-1 and two soluble IGF binding proteins with micromolar potency. NMR analysis revealed that the peptide is highly structured in the absence of IGF-1, and peptides that preorganize the binding elements were selected during the sorting.
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==About this Structure==
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Rapid identification of small binding motifs with high-throughput phage display: discovery of peptidic antagonists of IGF-1 function.,Deshayes K, Schaffer ML, Skelton NJ, Nakamura GR, Kadkhodayan S, Sidhu SS Chem Biol. 2002 Apr;9(4):495-505. PMID:11983338<ref>PMID:11983338</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB7 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Rapid identification of small binding motifs with high-throughput phage display: discovery of peptidic antagonists of IGF-1 function., Deshayes K, Schaffer ML, Skelton NJ, Nakamura GR, Kadkhodayan S, Sidhu SS, Chem Biol. 2002 Apr;9(4):495-505. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11983338 11983338]
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</div>
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[[Category: Deshayes, K.]]
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<div class="pdbe-citations 1lb7" style="background-color:#fffaf0;"></div>
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[[Category: Kadkhodayan, S.]]
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== References ==
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[[Category: Nakamura, G R.]]
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<references/>
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[[Category: Schaffer, M L.]]
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__TOC__
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[[Category: Sidhu, S S.]]
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</StructureSection>
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[[Category: Skelton, N J.]]
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[[Category: Large Structures]]
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[[Category: Disulfide]]
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[[Category: Deshayes K]]
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[[Category: Loop-helix]]
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[[Category: Kadkhodayan S]]
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[[Category: Peptide]]
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[[Category: Nakamura GR]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:44:46 2008''
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[[Category: Schaffer ML]]
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[[Category: Sidhu SS]]
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[[Category: Skelton NJ]]

Current revision

IGF-F1-1, A PEPTIDE ANTAGONIST OF IGF-1

PDB ID 1lb7

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