1mfk

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(New page: 200px<br /><applet load="1mfk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mfk" /> '''Structure of Prokaryotic SECIS mRNA Hairpin'...)
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[[Image:1mfk.gif|left|200px]]<br /><applet load="1mfk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mfk" />
 
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'''Structure of Prokaryotic SECIS mRNA Hairpin'''<br />
 
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==Overview==
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==Structure of Prokaryotic SECIS mRNA Hairpin==
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In prokaryotes, the recoding of a UGA stop codon as a selenocysteine codon, requires a special elongation factor (EF) SelB and a stem-loop structure, within the mRNA called a selenocysteine insertion sequence (SECIS). Here, we used NMR spectroscopy to determine the solution structure of the SECIS, mRNA hairpin and characterized its interaction with the mRNA-binding, domain of SelB. Our structural and biochemical data identified the, conserved structural features important for binding to EF SelB within, different SECIS RNA sequences. In the free SECIS mRNA structure, conserved, nucleotides are strongly exposed for recognition by SelB. Binding of the, C-terminal domain of SelB stabilizes the RNA secondary structure. In the, protein-RNA complex, a Watson-Crick loop base-pair leaves a GpU sequence, accessible for SelB recognition. This GpU sequence at the tip of the, capping tetraloop and a bulge uracil five Watson-Crick base-pairs apart, from the GpU are essential for interaction with SelB.
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<StructureSection load='1mfk' size='340' side='right'caption='[[1mfk]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mfk]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MFK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mfk OCA], [https://pdbe.org/1mfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mfk RCSB], [https://www.ebi.ac.uk/pdbsum/1mfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mfk ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In prokaryotes, the recoding of a UGA stop codon as a selenocysteine codon requires a special elongation factor (EF) SelB and a stem-loop structure within the mRNA called a selenocysteine insertion sequence (SECIS). Here, we used NMR spectroscopy to determine the solution structure of the SECIS mRNA hairpin and characterized its interaction with the mRNA-binding domain of SelB. Our structural and biochemical data identified the conserved structural features important for binding to EF SelB within different SECIS RNA sequences. In the free SECIS mRNA structure, conserved nucleotides are strongly exposed for recognition by SelB. Binding of the C-terminal domain of SelB stabilizes the RNA secondary structure. In the protein-RNA complex, a Watson-Crick loop base-pair leaves a GpU sequence accessible for SelB recognition. This GpU sequence at the tip of the capping tetraloop and a bulge uracil five Watson-Crick base-pairs apart from the GpU are essential for interaction with SelB.
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==About this Structure==
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Structure of prokaryotic SECIS mRNA hairpin and its interaction with elongation factor SelB.,Fourmy D, Guittet E, Yoshizawa S J Mol Biol. 2002 Nov 15;324(1):137-50. PMID:12421564<ref>PMID:12421564</ref>
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1MFK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MFK OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of prokaryotic SECIS mRNA hairpin and its interaction with elongation factor SelB., Fourmy D, Guittet E, Yoshizawa S, J Mol Biol. 2002 Nov 15;324(1):137-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12421564 12421564]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1mfk" style="background-color:#fffaf0;"></div>
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[[Category: Fourmy, D.]]
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== References ==
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[[Category: Guittet, E.]]
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<references/>
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[[Category: Yoshizawa, S.]]
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__TOC__
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[[Category: (a/u)gnn tetraloop family]]
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</StructureSection>
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[[Category: rna tetraloop]]
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[[Category: Large Structures]]
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[[Category: selb]]
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[[Category: Fourmy D]]
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[[Category: Guittet E]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:10:23 2007''
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[[Category: Yoshizawa S]]

Current revision

Structure of Prokaryotic SECIS mRNA Hairpin

PDB ID 1mfk

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