1mfk

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[[Image:1mfk.gif|left|200px]]
 
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==Structure of Prokaryotic SECIS mRNA Hairpin==
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The line below this paragraph, containing "STRUCTURE_1mfk", creates the "Structure Box" on the page.
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<StructureSection load='1mfk' size='340' side='right'caption='[[1mfk]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mfk]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MFK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mfk OCA], [https://pdbe.org/1mfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mfk RCSB], [https://www.ebi.ac.uk/pdbsum/1mfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mfk ProSAT]</span></td></tr>
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{{STRUCTURE_1mfk| PDB=1mfk | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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'''Structure of Prokaryotic SECIS mRNA Hairpin'''
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== Publication Abstract from PubMed ==
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==Overview==
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In prokaryotes, the recoding of a UGA stop codon as a selenocysteine codon requires a special elongation factor (EF) SelB and a stem-loop structure within the mRNA called a selenocysteine insertion sequence (SECIS). Here, we used NMR spectroscopy to determine the solution structure of the SECIS mRNA hairpin and characterized its interaction with the mRNA-binding domain of SelB. Our structural and biochemical data identified the conserved structural features important for binding to EF SelB within different SECIS RNA sequences. In the free SECIS mRNA structure, conserved nucleotides are strongly exposed for recognition by SelB. Binding of the C-terminal domain of SelB stabilizes the RNA secondary structure. In the protein-RNA complex, a Watson-Crick loop base-pair leaves a GpU sequence accessible for SelB recognition. This GpU sequence at the tip of the capping tetraloop and a bulge uracil five Watson-Crick base-pairs apart from the GpU are essential for interaction with SelB.
In prokaryotes, the recoding of a UGA stop codon as a selenocysteine codon requires a special elongation factor (EF) SelB and a stem-loop structure within the mRNA called a selenocysteine insertion sequence (SECIS). Here, we used NMR spectroscopy to determine the solution structure of the SECIS mRNA hairpin and characterized its interaction with the mRNA-binding domain of SelB. Our structural and biochemical data identified the conserved structural features important for binding to EF SelB within different SECIS RNA sequences. In the free SECIS mRNA structure, conserved nucleotides are strongly exposed for recognition by SelB. Binding of the C-terminal domain of SelB stabilizes the RNA secondary structure. In the protein-RNA complex, a Watson-Crick loop base-pair leaves a GpU sequence accessible for SelB recognition. This GpU sequence at the tip of the capping tetraloop and a bulge uracil five Watson-Crick base-pairs apart from the GpU are essential for interaction with SelB.
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==About this Structure==
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Structure of prokaryotic SECIS mRNA hairpin and its interaction with elongation factor SelB.,Fourmy D, Guittet E, Yoshizawa S J Mol Biol. 2002 Nov 15;324(1):137-50. PMID:12421564<ref>PMID:12421564</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFK OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of prokaryotic SECIS mRNA hairpin and its interaction with elongation factor SelB., Fourmy D, Guittet E, Yoshizawa S, J Mol Biol. 2002 Nov 15;324(1):137-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12421564 12421564]
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</div>
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[[Category: Fourmy, D.]]
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<div class="pdbe-citations 1mfk" style="background-color:#fffaf0;"></div>
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[[Category: Guittet, E.]]
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== References ==
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[[Category: Yoshizawa, S.]]
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<references/>
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[[Category: Rna tetraloop]]
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__TOC__
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[[Category: Selb]]
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</StructureSection>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:58:12 2008''
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[[Category: Large Structures]]
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[[Category: Fourmy D]]
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[[Category: Guittet E]]
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[[Category: Yoshizawa S]]

Current revision

Structure of Prokaryotic SECIS mRNA Hairpin

PDB ID 1mfk

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