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3etu

From Proteopedia

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Crystal structure of yeast Dsl1p

Structural highlights

3etu is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.4Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DSL1_YEAST Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Multisubunit tethering complexes are essential for intracellular trafficking and have been proposed to mediate the initial interaction between vesicles and the membranes with which they fuse. Here we report initial structural characterization of the Dsl1p complex, whose three subunits are essential for trafficking from the Golgi apparatus to the endoplasmic reticulum (ER). Crystal structures reveal that two of the three subunits, Tip20p and Dsl1p, resemble known subunits of the exocyst complex, establishing a structural connection among several multisubunit tethering complexes and implying that many of their subunits are derived from a common progenitor. We show, moreover, that Tip20p and Dsl1p interact directly via N-terminal alpha-helices. Finally, we establish that different Dsl1p complex subunits bind independently to different ER SNARE proteins. Our results map out two alternative protein-interaction networks capable of tethering COPI-coated vesicles, via the Dsl1p complex, to ER membranes.

Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex.,Tripathi A, Ren Y, Jeffrey PD, Hughson FM Nat Struct Mol Biol. 2009 Feb;16(2):114-23. Epub 2009 Jan 18. PMID:19151722^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vanrheenen SM, Reilly BA, Chamberlain SJ, Waters MG. Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast. Traffic. 2001 Mar;2(3):212-31. PMID:11260526
↑ Andag U, Neumann T, Schmitt HD. The coatomer-interacting protein Dsl1p is required for Golgi-to-endoplasmic reticulum retrieval in yeast. J Biol Chem. 2001 Oct 19;276(42):39150-60. Epub 2001 Aug 7. PMID:11493604 doi:http://dx.doi.org/10.1074/jbc.M105833200
↑ Reilly BA, Kraynack BA, VanRheenen SM, Waters MG. Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit. Mol Biol Cell. 2001 Dec;12(12):3783-96. PMID:11739780
↑ Tripathi A, Ren Y, Jeffrey PD, Hughson FM. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol. 2009 Feb;16(2):114-23. Epub 2009 Jan 18. PMID:19151722 doi:10.1038/nsmb.1548

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3etu"

Categories: Large Structures | Saccharomyces cerevisiae | Hughson FM | Jeffrey PD | Ren Y

@@ Line 1: / Line 1: @@
 ==Crystal structure of yeast Dsl1p==
-<StructureSection load='3etu' size='340' side='right' caption='[[3etu]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+<StructureSection load='3etu' size='340' side='right'caption='[[3etu]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3etu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ETU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3etu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ETU FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3etv|3etv]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DSL1, N0842, YNL258C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3etu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3etu OCA], [https://pdbe.org/3etu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3etu RCSB], [https://www.ebi.ac.uk/pdbsum/3etu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3etu ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3etu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3etu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3etu RCSB], [http://www.ebi.ac.uk/pdbsum/3etu PDBsum]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/DSL1_YEAST DSL1_YEAST] Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.<ref>PMID:11260526</ref> <ref>PMID:11493604</ref> <ref>PMID:11739780</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3etu" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Hughson, F M]]
+[[Category: Hughson FM]]
-[[Category: Jeffrey, P D]]
+[[Category: Jeffrey PD]]
-[[Category: Ren, Y]]
+[[Category: Ren Y]]
-[[Category: Endoplasmic reticulum]]
-[[Category: Er-golgi transport]]
-[[Category: Helical bundle]]
-[[Category: Membrane]]
-[[Category: Phosphoprotein]]
-[[Category: Protein transport]]
-[[Category: Transport]]
-[[Category: Transport protein]]

3etu

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Current revision

Crystal structure of yeast Dsl1p

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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