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| | ==Crystal structure of yeast Dsl1p== | | ==Crystal structure of yeast Dsl1p== |
| - | <StructureSection load='3etu' size='340' side='right' caption='[[3etu]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='3etu' size='340' side='right'caption='[[3etu]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3etu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ETU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3etu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ETU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ETU FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3etv|3etv]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DSL1, N0842, YNL258C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3etu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3etu OCA], [https://pdbe.org/3etu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3etu RCSB], [https://www.ebi.ac.uk/pdbsum/3etu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3etu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3etu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3etu OCA], [http://pdbe.org/3etu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3etu RCSB], [http://www.ebi.ac.uk/pdbsum/3etu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3etu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DSL1_YEAST DSL1_YEAST]] Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.<ref>PMID:11260526</ref> <ref>PMID:11493604</ref> <ref>PMID:11739780</ref> | + | [https://www.uniprot.org/uniprot/DSL1_YEAST DSL1_YEAST] Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.<ref>PMID:11260526</ref> <ref>PMID:11493604</ref> <ref>PMID:11739780</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Hughson, F M]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Jeffrey, P D]] | + | [[Category: Hughson FM]] |
| - | [[Category: Ren, Y]] | + | [[Category: Jeffrey PD]] |
| - | [[Category: Endoplasmic reticulum]] | + | [[Category: Ren Y]] |
| - | [[Category: Er-golgi transport]]
| + | |
| - | [[Category: Helical bundle]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Transport protein]]
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| Structural highlights
Function
DSL1_YEAST Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.[1] [2] [3]
Publication Abstract from PubMed
Multisubunit tethering complexes are essential for intracellular trafficking and have been proposed to mediate the initial interaction between vesicles and the membranes with which they fuse. Here we report initial structural characterization of the Dsl1p complex, whose three subunits are essential for trafficking from the Golgi apparatus to the endoplasmic reticulum (ER). Crystal structures reveal that two of the three subunits, Tip20p and Dsl1p, resemble known subunits of the exocyst complex, establishing a structural connection among several multisubunit tethering complexes and implying that many of their subunits are derived from a common progenitor. We show, moreover, that Tip20p and Dsl1p interact directly via N-terminal alpha-helices. Finally, we establish that different Dsl1p complex subunits bind independently to different ER SNARE proteins. Our results map out two alternative protein-interaction networks capable of tethering COPI-coated vesicles, via the Dsl1p complex, to ER membranes.
Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex.,Tripathi A, Ren Y, Jeffrey PD, Hughson FM Nat Struct Mol Biol. 2009 Feb;16(2):114-23. Epub 2009 Jan 18. PMID:19151722[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vanrheenen SM, Reilly BA, Chamberlain SJ, Waters MG. Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast. Traffic. 2001 Mar;2(3):212-31. PMID:11260526
- ↑ Andag U, Neumann T, Schmitt HD. The coatomer-interacting protein Dsl1p is required for Golgi-to-endoplasmic reticulum retrieval in yeast. J Biol Chem. 2001 Oct 19;276(42):39150-60. Epub 2001 Aug 7. PMID:11493604 doi:http://dx.doi.org/10.1074/jbc.M105833200
- ↑ Reilly BA, Kraynack BA, VanRheenen SM, Waters MG. Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit. Mol Biol Cell. 2001 Dec;12(12):3783-96. PMID:11739780
- ↑ Tripathi A, Ren Y, Jeffrey PD, Hughson FM. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol. 2009 Feb;16(2):114-23. Epub 2009 Jan 18. PMID:19151722 doi:10.1038/nsmb.1548
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