1oq0
From Proteopedia
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'''P6.1 stem loop from the activation domain of hTR''' | '''P6.1 stem loop from the activation domain of hTR''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OQ0 OCA]. | |
==Reference== | ==Reference== | ||
The solution structure of an essential stem-loop of human telomerase RNA., Leeper T, Leulliot N, Varani G, Nucleic Acids Res. 2003 May 15;31(10):2614-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12736311 12736311] | The solution structure of an essential stem-loop of human telomerase RNA., Leeper T, Leulliot N, Varani G, Nucleic Acids Res. 2003 May 15;31(10):2614-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12736311 12736311] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Leeper, T.]] | [[Category: Leeper, T.]] | ||
[[Category: Leulliot, N.]] | [[Category: Leulliot, N.]] | ||
[[Category: Varani, G.]] | [[Category: Varani, G.]] | ||
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Ribonucleoprotein]] |
| - | [[Category: | + | [[Category: Rna structure]] |
| - | [[Category: | + | [[Category: Telomerase]] |
| - | [[Category: | + | [[Category: Telomere]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:08:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:08, 3 May 2008
P6.1 stem loop from the activation domain of hTR
Overview
The ribonucleoprotein enzyme telomerase maintains chromosome ends in most eukaryotes and is critical for a cell's genetic stability and its proliferative viability. All telomerases contain a catalytic protein component homologous to viral reverse transcriptases (TERT) and an RNA (TR) that provides the template sequence as well as a scaffold for ribonucleoprotein assembly. Vertebrate telomerase RNAs have three essential domains: the template, activation and stability domains. Here we report the NMR structure of an essential RNA element derived from the human telomerase RNA activation domain. The sequence forms a stem-loop structure stabilized by a GU wobble pair formed by two of the five unpaired residues capping a short double helical region. The remaining three loop residues are in a well-defined conformation and form phosphate-base stacking interactions reminiscent of other RNA loop structures. Mutations of these unpaired nucleotides abolish enzymatic activity. The structure rationalizes a number of biochemical observations, and allows us to propose how the loop may function in the telomerase catalytic cycle. The pre-formed structure of the loop exposes the bases of these three essential nucleotides and positions them to interact with other RNA sequences within TR, with the reverse transcriptase or with the newly synthesized telomeric DNA strand. The functional role of this stem-loop appears to be conserved in even distantly related organisms such as yeast and ciliates.
About this Structure
Full crystallographic information is available from OCA.
Reference
The solution structure of an essential stem-loop of human telomerase RNA., Leeper T, Leulliot N, Varani G, Nucleic Acids Res. 2003 May 15;31(10):2614-21. PMID:12736311 Page seeded by OCA on Sat May 3 04:08:37 2008
