1dy2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1dy2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dy2, resolution 2.00&Aring;" /> '''MURINE COLLAGEN ALPH...)
Current revision (08:09, 6 December 2023) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1dy2.gif|left|200px]]<br /><applet load="1dy2" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1dy2, resolution 2.00&Aring;" />
 
-
'''MURINE COLLAGEN ALPHA1(XV), ENDOSTATIN DOMAIN'''<br />
 
-
==Overview==
+
==Murine collagen alpha1(XV), endostatin domain==
-
Endostatin is a fragment of the C-terminal domain NC1 of collagen XVIII, that inhibits angiogenesis and tumor growth. We report the, characterization of a collagen XV endostatin analogue and its parent NC1, domain, obtained by recombinant expression in mammalian cells. Both NC1, domains contain a trimerization domain, a hinge region that is more, sensitive to proteolysis in collagen XVIII and the endostatin domain., Unlike endostatin-XVIII, endostatin-XV does not bind zinc or heparin, which is explained by the crystal structure of endostatin-XV. The collagen, XV and XVIII fragments inhibited chorioallantoic membrane angiogenesis, induced by basic fibroblast growth factor (FGF-2) or vascular endothelial, growth factor (VEGF), but there are striking differences depending on, which cytokine is used and whether free endostatins or NC1 domains are, applied. The collagen XV and XVIII fragments showed a similar binding, repertoire for extracellular matrix proteins. Differences were found in, the immunohistological localization in vessel walls and basement membrane, zones. Together, these data indentify endostatin-XV as an angiogenesis, inhibitor, which differs from endostatin-XVIII in several important, functional details.
+
<StructureSection load='1dy2' size='340' side='right'caption='[[1dy2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1dy2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DY2 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dy2 OCA], [https://pdbe.org/1dy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dy2 RCSB], [https://www.ebi.ac.uk/pdbsum/1dy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dy2 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/COFA1_MOUSE COFA1_MOUSE] Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle. Restin potently inhibits angiogenesis.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dy/1dy2_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dy2 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Endostatin is a fragment of the C-terminal domain NC1 of collagen XVIII that inhibits angiogenesis and tumor growth. We report the characterization of a collagen XV endostatin analogue and its parent NC1 domain, obtained by recombinant expression in mammalian cells. Both NC1 domains contain a trimerization domain, a hinge region that is more sensitive to proteolysis in collagen XVIII and the endostatin domain. Unlike endostatin-XVIII, endostatin-XV does not bind zinc or heparin, which is explained by the crystal structure of endostatin-XV. The collagen XV and XVIII fragments inhibited chorioallantoic membrane angiogenesis induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF), but there are striking differences depending on which cytokine is used and whether free endostatins or NC1 domains are applied. The collagen XV and XVIII fragments showed a similar binding repertoire for extracellular matrix proteins. Differences were found in the immunohistological localization in vessel walls and basement membrane zones. Together, these data indentify endostatin-XV as an angiogenesis inhibitor, which differs from endostatin-XVIII in several important functional details.
-
==About this Structure==
+
Endostatins derived from collagens XV and XVIII differ in structural and binding properties, tissue distribution and anti-angiogenic activity.,Sasaki T, Larsson H, Tisi D, Claesson-Welsh L, Hohenester E, Timpl R J Mol Biol. 2000 Sep 1;301(5):1179-90. PMID:10966814<ref>PMID:10966814</ref>
-
1DY2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DY2 OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Endostatins derived from collagens XV and XVIII differ in structural and binding properties, tissue distribution and anti-angiogenic activity., Sasaki T, Larsson H, Tisi D, Claesson-Welsh L, Hohenester E, Timpl R, J Mol Biol. 2000 Sep 1;301(5):1179-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10966814 10966814]
+
</div>
-
[[Category: Mus musculus]]
+
<div class="pdbe-citations 1dy2" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Hohenester, E.]]
+
-
[[Category: Sasaki, T.]]
+
-
[[Category: Timpl, R.]]
+
-
[[Category: Tisi, D.]]
+
-
[[Category: SO4]]
+
-
[[Category: angiogenesis inhibitor]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:40:15 2007''
+
==See Also==
 +
*[[Collagen 3D structures|Collagen 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Mus musculus]]
 +
[[Category: Hohenester E]]
 +
[[Category: Sasaki T]]
 +
[[Category: Timpl R]]
 +
[[Category: Tisi D]]

Current revision

Murine collagen alpha1(XV), endostatin domain

PDB ID 1dy2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1dy2"
Personal tools