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1q7o
From Proteopedia
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| - | [[Image:1q7o.jpg|left|200px]]<br /><applet load="1q7o" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1q7o" /> | ||
| - | '''Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy'''<br /> | ||
| - | == | + | ==Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy== |
| + | <StructureSection load='1q7o' size='340' side='right'caption='[[1q7o]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1q7o]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q7O FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=MTY:META-TYROSINE'>MTY</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q7o OCA], [https://pdbe.org/1q7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q7o RCSB], [https://www.ebi.ac.uk/pdbsum/1q7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q7o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
The three-dimensional structure of the chemotactic peptide N-formyl-l-Met-l-Leu-l-Phe-OH was determined by using solid-state NMR (SSNMR). The set of SSNMR data consisted of 16 (13)C-(15)N distances and 18 torsion angle constraints (on 10 angles), recorded from uniformly (13)C,(15)N- and (15)N-labeled samples. The peptide's structure was calculated by means of simulated annealing and a newly developed protocol that ensures that all of conformational space, consistent with the structural constraints, is searched completely. The result is a high-quality structure of a molecule that has thus far not been amenable to single-crystal diffraction studies. The extensions of the SSNMR techniques and computational methods to larger systems appear promising. | The three-dimensional structure of the chemotactic peptide N-formyl-l-Met-l-Leu-l-Phe-OH was determined by using solid-state NMR (SSNMR). The set of SSNMR data consisted of 16 (13)C-(15)N distances and 18 torsion angle constraints (on 10 angles), recorded from uniformly (13)C,(15)N- and (15)N-labeled samples. The peptide's structure was calculated by means of simulated annealing and a newly developed protocol that ensures that all of conformational space, consistent with the structural constraints, is searched completely. The result is a high-quality structure of a molecule that has thus far not been amenable to single-crystal diffraction studies. The extensions of the SSNMR techniques and computational methods to larger systems appear promising. | ||
| - | + | De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy.,Rienstra CM, Tucker-Kellogg L, Jaroniec CP, Hohwy M, Reif B, McMahon MT, Tidor B, Lozano-Perez T, Griffin RG Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10260-5. Epub 2002 Jul 29. PMID:12149447<ref>PMID:12149447</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1q7o" style="background-color:#fffaf0;"></div> |
| - | [[Category: Griffin | + | == References == |
| - | [[Category: Hohwy | + | <references/> |
| - | [[Category: Jaroniec | + | __TOC__ |
| - | [[Category: Lozano-Perez | + | </StructureSection> |
| - | [[Category: McMahon | + | [[Category: Large Structures]] |
| - | [[Category: Reif | + | [[Category: Griffin RG]] |
| - | [[Category: Rienstra | + | [[Category: Hohwy M]] |
| - | [[Category: Tidor | + | [[Category: Jaroniec CP]] |
| - | [[Category: Tucker-Kellogg | + | [[Category: Lozano-Perez T]] |
| - | + | [[Category: McMahon MT]] | |
| - | + | [[Category: Reif B]] | |
| - | + | [[Category: Rienstra CM]] | |
| - | + | [[Category: Tidor B]] | |
| + | [[Category: Tucker-Kellogg L]] | ||
Current revision
Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy
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