1wks

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:28, 6 December 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1wks.gif|left|200px]]
 
-
<!--
+
==Solution structure of an RNA stem-loop derived from the 3' conserved region of eel LINE UnaL2==
-
The line below this paragraph, containing "STRUCTURE_1wks", creates the "Structure Box" on the page.
+
<StructureSection load='1wks' size='340' side='right'caption='[[1wks]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1wks]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WKS FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wks OCA], [https://pdbe.org/1wks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wks RCSB], [https://www.ebi.ac.uk/pdbsum/1wks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wks ProSAT]</span></td></tr>
-
{{STRUCTURE_1wks| PDB=1wks | SCENE= }}
+
</table>
-
 
+
<div style="background-color:#fffaf0;">
-
'''Solution structure of an RNA stem-loop derived from the 3' conserved region of eel LINE UnaL2'''
+
== Publication Abstract from PubMed ==
-
 
+
-
 
+
-
==Overview==
+
The eel long interspersed element (LINE) UnaL2 and its partner short interspersed element (SINE) share a conserved 3' tail containing a stem-loop that is critical for their retrotransposition. Presumably, the first step of retrotransposition is the recognition of their 3' tails by UnaL2-encoded reverse transcriptase. The solution structure of a 17-nucleotide RNA derived from the 3' tail of UnaL2 was determined by NMR. The GGAUA loop forms a specific structure in which the uridine is exposed to solvent with the third and fifth adenosines stacked. A sharp turn in the phosphodiester backbone occurs between the second guanosine and third adenosine. When the uridine is mutated (but not deleted), all mutants form the loop structure, indicating that the loop structure requires an exposed fourth residue. The retrotransposition assay in HeLa cells revealed that retrotransposition requires the second guanosine, although any nucleoside functions at the fourth position, suggesting that UnaL2 reverse transcriptase specifically recognizes the 5' side of the GGANA loop.
The eel long interspersed element (LINE) UnaL2 and its partner short interspersed element (SINE) share a conserved 3' tail containing a stem-loop that is critical for their retrotransposition. Presumably, the first step of retrotransposition is the recognition of their 3' tails by UnaL2-encoded reverse transcriptase. The solution structure of a 17-nucleotide RNA derived from the 3' tail of UnaL2 was determined by NMR. The GGAUA loop forms a specific structure in which the uridine is exposed to solvent with the third and fifth adenosines stacked. A sharp turn in the phosphodiester backbone occurs between the second guanosine and third adenosine. When the uridine is mutated (but not deleted), all mutants form the loop structure, indicating that the loop structure requires an exposed fourth residue. The retrotransposition assay in HeLa cells revealed that retrotransposition requires the second guanosine, although any nucleoside functions at the fourth position, suggesting that UnaL2 reverse transcriptase specifically recognizes the 5' side of the GGANA loop.
-
==About this Structure==
+
Solution structure of an RNA stem-loop derived from the 3' conserved region of eel LINE UnaL2.,Baba S, Kajikawa M, Okada N, Kawai G RNA. 2004 Sep;10(9):1380-7. Epub 2004 Jul 23. PMID:15273327<ref>PMID:15273327</ref>
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKS OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Solution structure of an RNA stem-loop derived from the 3' conserved region of eel LINE UnaL2., Baba S, Kajikawa M, Okada N, Kawai G, RNA. 2004 Sep;10(9):1380-7. Epub 2004 Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15273327 15273327]
+
</div>
-
[[Category: Baba, S.]]
+
<div class="pdbe-citations 1wks" style="background-color:#fffaf0;"></div>
-
[[Category: Kajikawa, M.]]
+
== References ==
-
[[Category: Kawai, G.]]
+
<references/>
-
[[Category: Okada, N.]]
+
__TOC__
-
[[Category: Line]]
+
</StructureSection>
-
[[Category: Nmr]]
+
[[Category: Large Structures]]
-
[[Category: Pentaloop]]
+
[[Category: Baba S]]
-
[[Category: Retrotransposition]]
+
[[Category: Kajikawa M]]
-
[[Category: Rna]]
+
[[Category: Kawai G]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:49:03 2008''
+
[[Category: Okada N]]

Current revision

Solution structure of an RNA stem-loop derived from the 3' conserved region of eel LINE UnaL2

PDB ID 1wks

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wks"
Personal tools