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1z2j

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{{Seed}}
 
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[[Image:1z2j.png|left|200px]]
 
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==Solution structure of the HIV-1 frameshift inducing element==
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The line below this paragraph, containing "STRUCTURE_1z2j", creates the "Structure Box" on the page.
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<StructureSection load='1z2j' size='340' side='right'caption='[[1z2j]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1z2j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z2J FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z2j OCA], [https://pdbe.org/1z2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z2j RCSB], [https://www.ebi.ac.uk/pdbsum/1z2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z2j ProSAT]</span></td></tr>
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{{STRUCTURE_1z2j| PDB=1z2j | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Expression of the HIV reverse transcriptase and other essential viral enzymes requires a -1 translational frameshift. The frameshift event is induced by two highly conserved RNA elements within the HIV-1 mRNA: a UUUUUUA heptamer known as the slippery sequence, and a downstream RNA structure. Here, we report structural and thermodynamic evidence that the HIV-1 frameshift site RNA forms a stem-loop and lower helix separated by a three-purine bulge. We have determined the structure of the 45 nucleotide frameshift site RNA using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The upper helix is highly thermostable (T(m)&gt;90 degrees C), forming 11 Watson-Crick base-pairs capped by a stable ACAA tetraloop. The eight base-pair lower helix was found to be only moderately stable (T(m)=47 degrees C). A three-purine bulge separates the highly stable upper helix from the lower helix. Base stacking in the bulge forms a wedge, introducing a 60 degrees bend between the helices. Interestingly, this bend is similar to those seen in a number of frameshift inducing pseudoknots for which structures have been solved. The lower helix must denature to allow the ribosome access to the slippery site, but likely functions as a positioning element that enhances frameshift efficiency.
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===Solution structure of the HIV-1 frameshift inducing element===
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Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element.,Staple DW, Butcher SE J Mol Biol. 2005 Jun 24;349(5):1011-23. Epub 2005 Apr 1. PMID:15927637<ref>PMID:15927637</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15927637}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1z2j" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15927637 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15927637}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Human immunodeficiency virus 1]]
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA].
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[[Category: Large Structures]]
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[[Category: Butcher SE]]
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==Reference==
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[[Category: Staple DW]]
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Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element., Staple DW, Butcher SE, J Mol Biol. 2005 Jun 24;349(5):1011-23. Epub 2005 Apr 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15927637 15927637]
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[[Category: Butcher, S E.]]
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[[Category: Staple, D W.]]
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[[Category: Purine bulge]]
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[[Category: Stem-loop]]
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[[Category: Tetraloop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:44:00 2008''
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Current revision

Solution structure of the HIV-1 frameshift inducing element

PDB ID 1z2j

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