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5oar
From Proteopedia
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==Crystal structure of native beta-N-acetylhexosaminidase isolated from Aspergillus oryzae== | ==Crystal structure of native beta-N-acetylhexosaminidase isolated from Aspergillus oryzae== | ||
| - | <StructureSection load='5oar' size='340' side='right' caption='[[5oar]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='5oar' size='340' side='right'caption='[[5oar]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5oar]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[5oar]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OAR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGT:3AR,5R,6S,7R,7AR-5-HYDROXYMETHYL-2-METHYL-5,6,7,7A-TETRAHYDRO-3AH-PYRANO[3,2-D]THIAZOLE-6,7-DIOL'>NGT</scene>, <scene name='pdbligand=TBR:HEXATANTALUM+DODECABROMIDE'>TBR</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NGT:3AR,5R,6S,7R,7AR-5-HYDROXYMETHYL-2-METHYL-5,6,7,7A-TETRAHYDRO-3AH-PYRANO[3,2-D]THIAZOLE-6,7-DIOL'>NGT</scene>, <scene name='pdbligand=TBR:HEXATANTALUM+DODECABROMIDE'>TBR</scene></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oar OCA], [https://pdbe.org/5oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oar RCSB], [https://www.ebi.ac.uk/pdbsum/5oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oar ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HEXA_ASPOZ HEXA_ASPOZ] Selectively hydrolyzes GlcNAcbeta(1->4)GlcNAc (N,N'-diacetylchitobiose) and Gal-NAcbeta(1->4)GlcNAc, but not their C-2 epimers GlcNAcbeta(1->4)ManNAc or Gal-NAcbeta(1->4)ManNAc. However, hydrolyzes both GlcNAcbeta(1->6)GlcNAc and GlcNAcbeta(1->6)ManNAc (Ref.7). Part of the binary chitinolytic system. Involved in hydrolyzation of chitobiose and higher chito-oligomers (produced from cell wall chitin by endochitinases), thus contributing to the formation of germ tubes, fruit-bodies and septa during hyphenation (Probable). Hydrolyzes synthetic substrate p-nitrophenyl-beta-N-acetyl-D-glucosaminide (pNP-GlcNAc) (PubMed:17302431, PubMed:12723619, Ref.7). Hydrolyzes synthetic substrate p-nitrophenyl-beta-N-acetyl-D-galactosaminide (pNP-GalNAc) (PubMed:12723619). Hydrolyzes chromogenic substrate 4-nitrophenyl-2-acetamido-2-deoxyglucopyranoside (PubMed:21505251, PubMed:17509134, PubMed:29239122).<ref>PMID:12723619</ref> <ref>PMID:17302431</ref> <ref>PMID:17509134</ref> <ref>PMID:21505251</ref> <ref>PMID:29239122</ref> <ref>PMID:15494009</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5oar" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5oar" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Beta-Hexosaminidase|Beta-Hexosaminidase]] | ||
| + | *[[Beta-Hexosaminidase 3D structures|Beta-Hexosaminidase 3D structures]] | ||
| + | *[[Beta-N-acetylhexosaminidase 3D structures|Beta-N-acetylhexosaminidase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Aspergillus oryzae]] | [[Category: Aspergillus oryzae]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Brynda | + | [[Category: Brynda J]] |
| - | [[Category: Otwinowski | + | [[Category: Otwinowski Z]] |
| - | [[Category: Pachl | + | [[Category: Pachl P]] |
| - | [[Category: Rezacova | + | [[Category: Rezacova P]] |
| - | [[Category: Skerlova | + | [[Category: Skerlova J]] |
| - | [[Category: Vanek | + | [[Category: Vanek O]] |
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Current revision
Crystal structure of native beta-N-acetylhexosaminidase isolated from Aspergillus oryzae
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