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| | <StructureSection load='5obc' size='340' side='right'caption='[[5obc]], [[Resolution|resolution]] 2.07Å' scene=''> | | <StructureSection load='5obc' size='340' side='right'caption='[[5obc]], [[Resolution|resolution]] 2.07Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5obc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OBC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OBC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5obc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OBC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9Q8:tris(oxidaniumylidynemethyl)-tris(oxidanyl)ruthenium'>9Q8</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=RU2:pentakis(oxidaniumyl)-(oxidaniumylidynemethyl)ruthenium'>RU2</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9Q8:tris(oxidaniumylidynemethyl)-tris(oxidanyl)ruthenium'>9Q8</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=RU2:pentakis(oxidaniumyl)-(oxidaniumylidynemethyl)ruthenium'>RU2</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5obc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5obc OCA], [http://pdbe.org/5obc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5obc RCSB], [http://www.ebi.ac.uk/pdbsum/5obc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5obc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5obc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5obc OCA], [https://pdbe.org/5obc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5obc RCSB], [https://www.ebi.ac.uk/pdbsum/5obc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5obc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN]] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref> | + | [https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Bos taurus]] | | [[Category: Bos taurus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pancreatic ribonuclease]]
| + | [[Category: Ferraro G]] |
| - | [[Category: Ferraro, G]] | + | [[Category: Merlino A]] |
| - | [[Category: Merlino, A]] | + | [[Category: Pontillo N]] |
| - | [[Category: Pontillo, N]] | + | |
| - | [[Category: Co releasing molecule]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Protein-metallodrug interaction]]
| + | |
| Structural highlights
Function
RNAS1_BOVIN Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.[1]
Publication Abstract from PubMed
fac-[RuII(CO)3Cl2(N3-Imidazole)] (RuIIIM), fac-[RuII(CO)3Cl2(N3-methyl-imidazole)] (RuIIMIM) and fac-[RuII(CO)3Cl2(N3-methyl-benzimidazole)] (RuIIMBI) are three ruthenium based CO releasing molecules (Ru-CORMs) that are cytotoxic towards ovarian and colon carcinoma cell lines. Detailed structural information on the adducts formed upon reaction of RuIIIM and RuIIMIM with hen egg white lysozyme and of the three Ru-CORMs with bovine pancreatic ribonuclease is provided here by X-ray crystallography. Comparative analysis of seven crystal structures of these adducts allows one to delineate some general trends in the reactivity of these Ru-CORMs with proteins. Indeed, in all cases Ru-CORMs bind these model systems upon detachment of the azole ligand and concomitant coordination to a protein His or Asp residue. Apparently the three Ru-CORMs progressively dissociate losing azoles, chlorides, and one or two CO molecules. Data were compared with those reported in the literature for adducts of the same proteins with other Ru-CORMs and with in-solution data previously obtained on the same systems. These results are potentially useful for a better understanding of the chemistry, potential toxicity and mechanism of actions of these interesting Ru-CORMs and are helpful in defining the molecular mechanisms of CO release.
Ru-Based CO releasing molecules with azole ligands: interaction with proteins and the CO release mechanism disclosed by X-ray crystallography.,Pontillo N, Ferraro G, Messori L, Tamasi G, Merlino A Dalton Trans. 2017 Jul 25;46(29):9621-9629. doi: 10.1039/c7dt01991b. PMID:28702564[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT. Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 1995 Mar;8(3):261-73. PMID:7479688
- ↑ Pontillo N, Ferraro G, Messori L, Tamasi G, Merlino A. Ru-Based CO releasing molecules with azole ligands: interaction with proteins and the CO release mechanism disclosed by X-ray crystallography. Dalton Trans. 2017 Jul 25;46(29):9621-9629. doi: 10.1039/c7dt01991b. PMID:28702564 doi:http://dx.doi.org/10.1039/c7dt01991b
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