1gn2

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{{Seed}}
 
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[[Image:1gn2.png|left|200px]]
 
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==S123C mutant of the iron-superoxide dismutase from Mycobacterium tuberculosis.==
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The line below this paragraph, containing "STRUCTURE_1gn2", creates the "Structure Box" on the page.
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<StructureSection load='1gn2' size='340' side='right'caption='[[1gn2]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1gn2]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GN2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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{{STRUCTURE_1gn2| PDB=1gn2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gn2 OCA], [https://pdbe.org/1gn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gn2 RCSB], [https://www.ebi.ac.uk/pdbsum/1gn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gn2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SODF_MYCTU SODF_MYCTU] Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/1gn2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gn2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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With the aim of enhancing interactions involved in dimer formation, an intersubunit disulfide bridge was engineered in the superoxide dismutase enzyme of Mycobacterium tuberculosis. Ser-123 was chosen for mutation to cysteine since it resides at the dimer interface where the serine side chain interacts with the same residue in the opposite subunit. Gel electrophoresis and X-ray crystallographic studies of the expressed mutant confirmed formation of the disulfide bond under nonreducing conditions. However, the mutant protein was found to be less stable than the wild type as judged by susceptibility to denaturation in the presence of guanidine hydrochloride. Decreased stability probably results from formation of a disulfide bridge with a suboptimal torsion angle and exclusion of solvent molecules from the dimer interface.
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===S123C MUTANT OF THE IRON-SUPEROXIDE DISMUTASE FROM MYCOBACTERIUM TUBERCULOSIS.===
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Engineering of an intersubunit disulfide bridge in the iron-superoxide dismutase of Mycobacterium tuberculosis.,Bunting KA, Cooper JB, Tickle IJ, Young DB Arch Biochem Biophys. 2002 Jan 1;397(1):69-76. PMID:11747311<ref>PMID:11747311</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1gn2" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11747311}}, adds the Publication Abstract to the page
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*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11747311 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11747311}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1GN2 is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GN2 OCA].
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==Reference==
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<ref group="xtra">PMID:11747311</ref><references group="xtra"/>
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Superoxide dismutase]]
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[[Category: Bunting KA]]
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[[Category: Bunting, K A.]]
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[[Category: Cooper JB]]
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[[Category: Cooper, J B.]]
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[[Category: Tickle IJ]]
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[[Category: Tickle, I J.]]
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[[Category: Young DB]]
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[[Category: Young, D B.]]
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[[Category: Iron]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:09:44 2009''
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Current revision

S123C mutant of the iron-superoxide dismutase from Mycobacterium tuberculosis.

PDB ID 1gn2

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OCA

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