1gxq

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(New page: 200px<br /><applet load="1gxq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gxq, resolution 2.00&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1gxq.gif|left|200px]]<br /><applet load="1gxq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gxq, resolution 2.00&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE PHOB EFFECTOR DOMAIN'''<br />
 
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==Overview==
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==Crystal structure of the PhoB effector domain==
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PhoB is a signal transduction response regulator that activates nearly 40, genes in phosphate depletion conditions in E. coli and closely related, bacteria. The structure of the PhoB effector domain in complex with its, target DNA sequence, or pho box, reveals a novel tandem arrangement in, which several monomers bind head to tail to successive 11-base pair, direct-repeat sequences, coating one face of a smoothly bent double helix., The protein has a winged helix fold in which the DNA recognition elements, comprise helix alpha 3, penetrating the major groove, and a beta hairpin, wing interacting with a compressed minor groove via Arg219, tightly, sandwiched between the DNA sugar backbones. The transactivation loops, protrude laterally in an appropriate orientation to interact with the RNA, polymerase sigma(70) subunit, which triggers transcription initiation.
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<StructureSection load='1gxq' size='340' side='right'caption='[[1gxq]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gxq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GXQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gxq OCA], [https://pdbe.org/1gxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gxq RCSB], [https://www.ebi.ac.uk/pdbsum/1gxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gxq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHOB_ECOLI PHOB_ECOLI] This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/1gxq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gxq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PhoB is a signal transduction response regulator that activates nearly 40 genes in phosphate depletion conditions in E. coli and closely related bacteria. The structure of the PhoB effector domain in complex with its target DNA sequence, or pho box, reveals a novel tandem arrangement in which several monomers bind head to tail to successive 11-base pair direct-repeat sequences, coating one face of a smoothly bent double helix. The protein has a winged helix fold in which the DNA recognition elements comprise helix alpha 3, penetrating the major groove, and a beta hairpin wing interacting with a compressed minor groove via Arg219, tightly sandwiched between the DNA sugar backbones. The transactivation loops protrude laterally in an appropriate orientation to interact with the RNA polymerase sigma(70) subunit, which triggers transcription initiation.
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==About this Structure==
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Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator.,Blanco AG, Sola M, Gomis-Ruth FX, Coll M Structure. 2002 May;10(5):701-13. PMID:12015152<ref>PMID:12015152</ref>
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1GXQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GXQ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator., Blanco AG, Sola M, Gomis-Ruth FX, Coll M, Structure. 2002 May;10(5):701-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12015152 12015152]
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</div>
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<div class="pdbe-citations 1gxq" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Blanco, A.G.]]
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[[Category: Blanco AG]]
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[[Category: Coll, M.]]
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[[Category: Coll M]]
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[[Category: Gomis-Ruth, F.X.]]
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[[Category: Gomis-Ruth FX]]
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[[Category: Sola, M.]]
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[[Category: Sola M]]
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[[Category: activator]]
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[[Category: dna binding]]
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[[Category: helix-winged-helix]]
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[[Category: phosphorylation]]
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[[Category: sensory transduction]]
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[[Category: transcriptional activator]]
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[[Category: two-component signal transduction]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:20:14 2007''
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Current revision

Crystal structure of the PhoB effector domain

PDB ID 1gxq

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