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| | <StructureSection load='1h5q' size='340' side='right'caption='[[1h5q]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='1h5q' size='340' side='right'caption='[[1h5q]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1h5q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Agabi Agabi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H5Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1h5q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H5Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannitol_2-dehydrogenase_(NADP(+)) Mannitol 2-dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.138 1.1.1.138] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h5q OCA], [http://pdbe.org/1h5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h5q RCSB], [http://www.ebi.ac.uk/pdbsum/1h5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h5q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h5q OCA], [https://pdbe.org/1h5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h5q RCSB], [https://www.ebi.ac.uk/pdbsum/1h5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h5q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/MTDH_AGABI MTDH_AGABI] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </div> | | </div> |
| | <div class="pdbe-citations 1h5q" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 1h5q" style="background-color:#fffaf0;"></div> |
| - | | |
| - | ==See Also== | |
| - | *[[InhA|InhA]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Agabi]] | + | [[Category: Agaricus bisporus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Baumann, U]] | + | [[Category: Baumann U]] |
| - | [[Category: Horer, S]] | + | [[Category: Horer S]] |
| - | [[Category: Mooibroek, H]] | + | [[Category: Mooibroek H]] |
| - | [[Category: Sassoon, J]] | + | [[Category: Sassoon J]] |
| - | [[Category: Stoop, J]] | + | [[Category: Stoop J]] |
| - | [[Category: Mannitol metabolism]]
| + | |
| - | [[Category: Oxidoreductase]]
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| Structural highlights
Function
MTDH_AGABI
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Mannitol, an acyclic six-carbon polyol, is one of the most abundant sugar alcohols occurring in nature. In the button mushroom, Agaricus bisporus, it is synthesized from fructose by the enzyme mannitol 2-dehydrogenase (MtDH; EC ) using NADPH as a cofactor. Mannitol serves as the main storage carbon (up to 50% of the fruit body dry weight) and plays a critical role in growth, fruit body development, osmoregulation, and salt tolerance. Furthermore, mannitol dehydrogenases are being evaluated for commercial mannitol production as alternatives to the less efficient chemical reduction of fructose. Given the importance of mannitol metabolism and mannitol dehydrogenases, MtDH was cloned into the pET28 expression system and overexpressed in Escherichia coli. Kinetic and physicochemical properties of the recombinant enzyme are indistinguishable from the natural enzyme. The crystal structure of its binary complex with NADP was solved at 1.5-A resolution and refined to an R value of 19.3%. It shows MtDH to be a tetramer and a member of the short chain dehydrogenase/reductase family of enzymes. The catalytic residues forming the so-called catalytic triad can be assigned to Ser(149), Tyr(169), and Lys(173).
The crystallographic structure of the mannitol 2-dehydrogenase NADP+ binary complex from Agaricus bisporus.,Horer S, Stoop J, Mooibroek H, Baumann U, Sassoon J J Biol Chem. 2001 Jul 20;276(29):27555-61. Epub 2001 May 2. PMID:11335726[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Horer S, Stoop J, Mooibroek H, Baumann U, Sassoon J. The crystallographic structure of the mannitol 2-dehydrogenase NADP+ binary complex from Agaricus bisporus. J Biol Chem. 2001 Jul 20;276(29):27555-61. Epub 2001 May 2. PMID:11335726 doi:10.1074/jbc.M102850200
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