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1h6s

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Current revision

Asymmetric conductivity of engineered proteins

Structural highlights

1h6s is a 1 chain structure with sequence from Fuscovulum blasticum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PORI_FUSBL Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Positively charged peptide segments of 16 and 18 residues were inserted at a periplasmic turn of the porin from Rhodobacter blasticus in order to form an electric field-dependent plug. The X-ray diffraction analysis of a mutant confirmed that the structure of the porin had remained intact and that the insert was mobile. Incorporation experiments of single molecules into lipid bilayers showed that the distribution of electric conduction increments depended on the field polarity. The observed distributions are explained if the porin molecules enter the bilayer preferentially with their periplasmic surface first. Furthermore, the conduction of membrane-incorporated porin mutants changed reproducibly on field reversal showing asymmetries of reverse similar 15%, while the wild-type remained constant. This asymmetry is most likely caused by the electric field pressing the charged insert onto the pore eyelet in one field direction and removing it from the eyelet in the other. The results encourage attempts to improve the inserts in order to eventually reach diode characteristics.

Asymmetric conductivity of engineered porins.,Bannwarth M, Schulz GE Protein Eng. 2002 Oct;15(10):799-804. PMID:12468713^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bannwarth M, Schulz GE. Asymmetric conductivity of engineered porins. Protein Eng. 2002 Oct;15(10):799-804. PMID:12468713

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1h6s"

Categories: Fuscovulum blasticum | Large Structures | Bannwarth M | Schulz GE

@@ Line 3: / Line 3: @@
 <StructureSection load='1h6s' size='340' side='right'caption='[[1h6s]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1h6s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33485 Atcc 33485]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H6S FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1h6s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuscovulum_blasticum Fuscovulum blasticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H6S FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bh3|1bh3]], [[1prn|1prn]], [[2prn|2prn]], [[3prn|3prn]], [[5prn|5prn]], [[6prn|6prn]], [[7prn|7prn]], [[8prn|8prn]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h6s OCA], [https://pdbe.org/1h6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h6s RCSB], [https://www.ebi.ac.uk/pdbsum/1h6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h6s ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/PORI_RHOBL PORI_RHOBL]] Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.
+[https://www.uniprot.org/uniprot/PORI_FUSBL PORI_FUSBL] Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 33485]]
+[[Category: Fuscovulum blasticum]]
 [[Category: Large Structures]]
-[[Category: Bannwarth, M]]
+[[Category: Bannwarth M]]
-[[Category: Schulz, G E]]
+[[Category: Schulz GE]]
-[[Category: Integral membrane protein porin]]
-[[Category: Membrane protein]]

1h6s

From Proteopedia

Current revision

Asymmetric conductivity of engineered proteins

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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