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1hbx

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Ternary Complex of SAP-1 and SRF with specific SRE DNA

Structural highlights

1hbx is a 10 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.15Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SRF_HUMAN SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1.

The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex.,Hassler M, Richmond TJ EMBO J. 2001 Jun 15;20(12):3018-28. PMID:11406578^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hassler M, Richmond TJ. The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex. EMBO J. 2001 Jun 15;20(12):3018-28. PMID:11406578 doi:http://dx.doi.org/10.1093/emboj/20.12.3018

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hbx"

Categories: Homo sapiens | Large Structures | Hassler M | Richmond TJ

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:1hbx.png|left|200px]]
-<!--
+==Ternary Complex of SAP-1 and SRF with specific SRE DNA==
-The line below this paragraph, containing "STRUCTURE_1hbx", creates the "Structure Box" on the page.
+<StructureSection load='1hbx' size='340' side='right'caption='[[1hbx]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1hbx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HBX FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hbx OCA], [https://pdbe.org/1hbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hbx RCSB], [https://www.ebi.ac.uk/pdbsum/1hbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hbx ProSAT]</span></td></tr>
-{{STRUCTURE_1hbx|  PDB=1hbx  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SRF_HUMAN SRF_HUMAN] SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hb/1hbx_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hbx ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1.
-===TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA===
+The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex.,Hassler M, Richmond TJ EMBO J. 2001 Jun 15;20(12):3018-28. PMID:11406578<ref>PMID:11406578</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_11406578}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1hbx" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 11406578 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_11406578}}
+__TOC__
+</StructureSection>
-==About this Structure==
-HBX is a 10 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBX OCA].
-==Reference==
-<ref group="xtra">PMID:11406578</ref><references group="xtra"/>
 [[Category: Homo sapiens]]
-[[Category: Hassler, M.]]
+[[Category: Large Structures]]
-[[Category: Richmond, T J.]]
+[[Category: Hassler M]]
-[[Category: Gene regulation]]
+[[Category: Richmond TJ]]
-[[Category: Serum response factor]]
-[[Category: Ternary complex factor]]
-[[Category: Transcription complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 20:40:49 2009''

1hbx

From Proteopedia

Current revision

Ternary Complex of SAP-1 and SRF with specific SRE DNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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