1hg7

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[[Image:1hg7.jpg|left|200px]]
 
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{{Structure
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==High resolution structure of HPLC-12 type III antifreeze protein from Ocean Pout Macrozoarces americanus==
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|PDB= 1hg7 |SIZE=350|CAPTION= <scene name='initialview01'>1hg7</scene>, resolution 1.15&Aring;
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<StructureSection load='1hg7' size='340' side='right'caption='[[1hg7]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1hg7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HG7 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
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|GENE= RECOMBINANT TYPE III AFP HPURCE 10 FRACTION 12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8199 Macrozoarces americanus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hg7 OCA], [https://pdbe.org/1hg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hg7 RCSB], [https://www.ebi.ac.uk/pdbsum/1hg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hg7 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hg7 OCA], [http://www.ebi.ac.uk/pdbsum/1hg7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hg7 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ANP12_ZOAAM ANP12_ZOAAM] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.
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== Evolutionary Conservation ==
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'''HIGH RESOLUTION STRUCTURE OF HPLC-12 TYPE III ANTIFREEZE PROTEIN FROM OCEAN POUT MACROZOARCES AMERICANUS'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hg/1hg7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hg7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Type III antifreeze proteins (AFPs) are present in the body fluids of some polar fishes where they inhibit ice growth at subzero temperatures. Previous studies of the structure of type III AFP by NMR and X-ray identified a remarkably flat surface on the protein containing amino acids that were demonstrated to be important for interaction with ice by mutational studies. It was proposed that this protein surface binds onto the (1 0 [\bar 1] 0) plane of ice with the key amino acids interacting directly with the water molecules in the ice crystal. Here, we show that the mechanism of type III AFP interaction with ice crystals is more complex than that proposed previously. We report a high-resolution X-ray structure of type III AFP refined at 1.15 A resolution with individual anisotropic temperature factors. We report the results of ice-etching experiments that show a broad surface coverage, suggesting that type III AFP binds to a set of planes that are parallel with or inclined at a small angle to the crystallographic c-axis of the ice crystal. Our modelling studies, performed with the refined structure, confirm that type III AFP can make energetically favourable interactions with several ice surfaces.
Type III antifreeze proteins (AFPs) are present in the body fluids of some polar fishes where they inhibit ice growth at subzero temperatures. Previous studies of the structure of type III AFP by NMR and X-ray identified a remarkably flat surface on the protein containing amino acids that were demonstrated to be important for interaction with ice by mutational studies. It was proposed that this protein surface binds onto the (1 0 [\bar 1] 0) plane of ice with the key amino acids interacting directly with the water molecules in the ice crystal. Here, we show that the mechanism of type III AFP interaction with ice crystals is more complex than that proposed previously. We report a high-resolution X-ray structure of type III AFP refined at 1.15 A resolution with individual anisotropic temperature factors. We report the results of ice-etching experiments that show a broad surface coverage, suggesting that type III AFP binds to a set of planes that are parallel with or inclined at a small angle to the crystallographic c-axis of the ice crystal. Our modelling studies, performed with the refined structure, confirm that type III AFP can make energetically favourable interactions with several ice surfaces.
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==About this Structure==
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Understanding the mechanism of ice binding by type III antifreeze proteins.,Antson AA, Smith DJ, Roper DI, Lewis S, Caves LS, Verma CS, Buckley SL, Lillford PJ, Hubbard RE J Mol Biol. 2001 Jan 26;305(4):875-89. PMID:11162099<ref>PMID:11162099</ref>
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1HG7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Macrozoarces_americanus Macrozoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG7 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Understanding the mechanism of ice binding by type III antifreeze proteins., Antson AA, Smith DJ, Roper DI, Lewis S, Caves LS, Verma CS, Buckley SL, Lillford PJ, Hubbard RE, J Mol Biol. 2001 Jan 26;305(4):875-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11162099 11162099]
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</div>
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[[Category: Macrozoarces americanus]]
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<div class="pdbe-citations 1hg7" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Antson, A A.]]
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[[Category: Buckley, S L.]]
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[[Category: Caves, L S.D.]]
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[[Category: Hubbard, R E.]]
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[[Category: Lewis, S.]]
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[[Category: Lillford, P J.]]
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[[Category: Roper, D I.]]
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[[Category: Smith, D J.]]
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[[Category: Verma, C S.]]
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[[Category: antifreeze protein]]
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[[Category: ice-binding protein]]
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[[Category: macrozoarces americanus]]
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[[Category: ocean pout]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:04:25 2008''
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==See Also==
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*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Zoarces americanus]]
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[[Category: Antson AA]]
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[[Category: Buckley SL]]
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[[Category: Caves LSD]]
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[[Category: Hubbard RE]]
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[[Category: Lewis S]]
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[[Category: Lillford PJ]]
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[[Category: Roper DI]]
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[[Category: Smith DJ]]
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[[Category: Verma CS]]

Current revision

High resolution structure of HPLC-12 type III antifreeze protein from Ocean Pout Macrozoarces americanus

PDB ID 1hg7

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