1o7t

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[[Image:1o7t.gif|left|200px]]<br /><applet load="1o7t" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1o7t, resolution 1.65&Aring;" />
 
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'''METAL NANOCLUSTERS BOUND TO THE FERRIC BINDING PROTEIN FROM NEISSERIA GONORRHOEAE.'''<br />
 
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==Overview==
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==Metal nanoclusters bound to the Ferric Binding Protein from Neisseria gonorrhoeae.==
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Transferrins transport Fe3+ and other metal ions in mononuclear-binding, sites. We present the first evidence that a member of the transferrin, superfamily is able to recognize multi-nuclear oxo-metal clusters, small, mineral fragments that are the most abundant forms of many metals in the, environment. We show that the ferric ion-binding protein from Neisseria, gonorrhoeae (nFbp) readily binds clusters of Fe3+, Ti4+, Zr4+ or Hf4+ in, solution. The 1.7 A resolution crystal structure of Hf-nFbp reveals three, distinct types of clusters in an open, positively charged cleft between, two hinged protein domains. A di-tyrosyl cluster nucleation motif, (Tyr195-Tyr196) is situated at the bottom of this cleft and binds either a, trinuclear oxo-Hf cluster, which is capped by phosphate, or a pentanuclear, cluster, which in turn can be capped with phosphate. This first, high-resolution structure of a protein-mineral interface suggests a novel, metal-uptake mechanism and provides a model for protein-mediated, mineralization/dissimilation, which plays a critical role in geochemical, processes.
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<StructureSection load='1o7t' size='340' side='right'caption='[[1o7t]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1o7t]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HF3:SMALLEST+HF-OXO-PHOSPHATE+CLUSTER+HF3'>HF3</scene>, <scene name='pdbligand=HF5:HF+OXO+CLUSTER+HF5'>HF5</scene>, <scene name='pdbligand=PHF:HF-OXO-PHOSPHATE+CLUSTER+PHF'>PHF</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7t OCA], [https://pdbe.org/1o7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7t RCSB], [https://www.ebi.ac.uk/pdbsum/1o7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q50964_NEIGO Q50964_NEIGO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transferrins transport Fe3+ and other metal ions in mononuclear-binding sites. We present the first evidence that a member of the transferrin superfamily is able to recognize multi-nuclear oxo-metal clusters, small mineral fragments that are the most abundant forms of many metals in the environment. We show that the ferric ion-binding protein from Neisseria gonorrhoeae (nFbp) readily binds clusters of Fe3+, Ti4+, Zr4+ or Hf4+ in solution. The 1.7 A resolution crystal structure of Hf-nFbp reveals three distinct types of clusters in an open, positively charged cleft between two hinged protein domains. A di-tyrosyl cluster nucleation motif (Tyr195-Tyr196) is situated at the bottom of this cleft and binds either a trinuclear oxo-Hf cluster, which is capped by phosphate, or a pentanuclear cluster, which in turn can be capped with phosphate. This first high-resolution structure of a protein-mineral interface suggests a novel metal-uptake mechanism and provides a model for protein-mediated mineralization/dissimilation, which plays a critical role in geochemical processes.
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==About this Structure==
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A novel protein-mineral interface.,Alexeev D, Zhu H, Guo M, Zhong W, Hunter DJ, Yang W, Campopiano DJ, Sadler PJ Nat Struct Biol. 2003 Apr;10(4):297-302. PMID:12598891<ref>PMID:12598891</ref>
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1O7T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae] with <scene name='pdbligand=HF5:'>HF5</scene>, <scene name='pdbligand=HF3:'>HF3</scene> and <scene name='pdbligand=PHF:'>PHF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=HF1:Phf+Binding+Site+For+Chain+I'>HF1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7T OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A novel protein-mineral interface., Alexeev D, Zhu H, Guo M, Zhong W, Hunter DJ, Yang W, Campopiano DJ, Sadler PJ, Nat Struct Biol. 2003 Apr;10(4):297-302. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12598891 12598891]
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</div>
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[[Category: Neisseria gonorrhoeae]]
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<div class="pdbe-citations 1o7t" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Alexeev, D.]]
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[[Category: Campopiano, D.J.]]
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[[Category: Guo, M.]]
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[[Category: Hunter, D.J.B.]]
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[[Category: Sadler, P.J.]]
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[[Category: Yang, W.]]
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[[Category: Zhong, W.]]
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[[Category: Zu, H.]]
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[[Category: HF3]]
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[[Category: HF5]]
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[[Category: PHF]]
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[[Category: hafnium]]
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[[Category: metal-oxo cluster]]
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[[Category: periplasmic ferric binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:54:37 2008''
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==See Also==
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*[[Ferric-binding protein|Ferric-binding protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Neisseria gonorrhoeae]]
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[[Category: Alexeev D]]
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[[Category: Campopiano DJ]]
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[[Category: Guo M]]
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[[Category: Hunter DJB]]
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[[Category: Sadler PJ]]
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[[Category: Yang W]]
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[[Category: Zhong W]]
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[[Category: Zu H]]

Current revision

Metal nanoclusters bound to the Ferric Binding Protein from Neisseria gonorrhoeae.

PDB ID 1o7t

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