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1odz

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==Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides==
==Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides==
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<StructureSection load='1odz' size='340' side='right' caption='[[1odz]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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<StructureSection load='1odz' size='340' side='right'caption='[[1odz]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1odz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ODZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ODZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1odz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ODZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ODZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1odz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1odz OCA], [http://pdbe.org/1odz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1odz RCSB], [http://www.ebi.ac.uk/pdbsum/1odz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1odz ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1odz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1odz OCA], [https://pdbe.org/1odz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1odz RCSB], [https://www.ebi.ac.uk/pdbsum/1odz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1odz ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MANA_CELJU MANA_CELJU] Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.<ref>PMID:7848261</ref> <ref>PMID:11064195</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</div>
</div>
<div class="pdbe-citations 1odz" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 1odz" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Mannosidase 3D structures|Mannosidase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cellvibrio cellulosa nagy et al. 2002]]
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[[Category: Cellvibrio japonicus]]
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[[Category: Mannan endo-1,4-beta-mannosidase]]
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[[Category: Large Structures]]
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[[Category: Davies, G J]]
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[[Category: Davies GJ]]
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[[Category: Ducros, V M.A]]
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[[Category: Ducros VMA]]
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[[Category: Gilbert, H J]]
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[[Category: Gilbert HJ]]
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[[Category: Jahn, M]]
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[[Category: Jahn M]]
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[[Category: Singh, P]]
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[[Category: Singh P]]
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[[Category: Stoll, D]]
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[[Category: Stoll D]]
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[[Category: Szabo, L]]
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[[Category: Szabo L]]
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[[Category: Warren, R A.J]]
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[[Category: Warren RAJ]]
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[[Category: Withers, S G]]
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[[Category: Withers SG]]
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[[Category: Family 26]]
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[[Category: Glycoside-hydrolase]]
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[[Category: Glycosynthase]]
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[[Category: Hydrolase]]
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[[Category: Mannanase]]
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[[Category: Manno-oligosaccharide]]
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Current revision

Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides

PDB ID 1odz

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