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1ogv

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(New page: 200px<br /> <applet load="1ogv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ogv, resolution 2.35&Aring;" /> '''LIPIDIC CUBIC PHASE...)
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[[Image:1ogv.gif|left|200px]]<br />
 
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<applet load="1ogv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ogv, resolution 2.35&Aring;" />
 
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'''LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES'''<br />
 
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==Overview==
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==Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides==
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Well-ordered crystals of the bacterial photosynthetic reaction centre from, Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we, report the type I crystal packing that results from this crystallisation, medium, for which 3D crystals grow as stacked 2D crystals, and the, reaction centre X-ray structure is refined to 2.35A resolution. In this, crystal form, the location of the membrane bilayer could be assigned with, confidence. A cardiolipin-binding site is found at the protein-protein, interface within the membrane-spanning region, shedding light on the, formation of crystal contacts within the membrane. A chloride-binding site, was identified in the membrane-spanning region, which suggests a putative, site for interaction with the light-harvesting complex I, the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12899837 (full description)]]
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<StructureSection load='1ogv' size='340' side='right'caption='[[1ogv]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ogv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OGV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ogv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ogv OCA], [https://pdbe.org/1ogv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ogv RCSB], [https://www.ebi.ac.uk/pdbsum/1ogv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ogv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RCEH_CERSP RCEH_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/og/1ogv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ogv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Well-ordered crystals of the bacterial photosynthetic reaction centre from Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we report the type I crystal packing that results from this crystallisation medium, for which 3D crystals grow as stacked 2D crystals, and the reaction centre X-ray structure is refined to 2.35A resolution. In this crystal form, the location of the membrane bilayer could be assigned with confidence. A cardiolipin-binding site is found at the protein-protein interface within the membrane-spanning region, shedding light on the formation of crystal contacts within the membrane. A chloride-binding site was identified in the membrane-spanning region, which suggests a putative site for interaction with the light-harvesting complex I, the cytochrome bc(1) complex or PufX. Comparisons with the X-ray structures of this reaction centre deriving from detergent-based crystals are drawn, indicating that a slight compression occurs in this lipid-rich environment.
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==About this Structure==
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Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution.,Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:12899837<ref>PMID:12899837</ref>
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1OGV is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]] with FE2, CL, BCL, BPH, U10 and CDL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGV OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution., Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R, J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12899837 12899837]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1ogv" style="background-color:#fffaf0;"></div>
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[[Category: Rhodobacter sphaeroides]]
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== References ==
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[[Category: Andreasson, L.E.]]
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<references/>
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[[Category: Andreasson, U.]]
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__TOC__
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[[Category: Katona, G.]]
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</StructureSection>
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[[Category: Landau, E.M.]]
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[[Category: Cereibacter sphaeroides]]
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[[Category: Neutze, R.]]
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[[Category: Large Structures]]
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[[Category: BCL]]
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[[Category: Andreasson L-E]]
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[[Category: BPH]]
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[[Category: Andreasson U]]
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[[Category: CDL]]
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[[Category: Katona G]]
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[[Category: CL]]
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[[Category: Landau EM]]
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[[Category: FE2]]
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[[Category: Neutze R]]
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[[Category: U10]]
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[[Category: bacteriochlorophyll]]
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[[Category: charge separation]]
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[[Category: electron transport]]
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[[Category: integral membrane protein]]
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[[Category: lipid]]
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[[Category: photosynthesis]]
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[[Category: reaction centre]]
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[[Category: transmembrane]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:14:12 2007''
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Current revision

Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides

PDB ID 1ogv

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