1oi4

Structural highlights

Function

YHBO_ECOLI Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals (PubMed:26774339, PubMed:28596309). Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Is able to repair glycated serum albumin, collagen, glyceraldehyde-3-phosphate dehydrogenase, and fructose biphosphate aldolase. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (PubMed:26774339). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair (PubMed:28596309). In vitro, prevents acrylamide formation in asparagine/glyoxal and asparagine/sugar mixtures at 55 degrees Celsius, likely by degrading asparagine/glyoxal Maillard adducts formed at high temperatures (PubMed:27530919). Also displays an apparent glyoxalase activity that in fact reflects its deglycase activity (PubMed:26774339, PubMed:26678554). Is a general stress protein; is required for the protection of bacterial cells against many environmental stresses, including oxidative, thermal, osmotic, UV, and pH stresses (PubMed:17933887). And plays an important role in protection against electrophile/carbonyl stress (PubMed:26774339).^{[1] [2] [3] [4] [5]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Abdallah J, Caldas T, Kthiri F, Kern R, Richarme G. YhbO protects cells against multiple stresses. J Bacteriol. 2007 Dec;189(24):9140-4. PMID:17933887 doi:10.1128/JB.01208-07
2. ↑ Lee C, Lee J, Lee JY, Park C. Characterization of the Escherichia coli YajL, YhbO and ElbB glyoxalases. FEMS Microbiol Lett. 2016 Feb;363(3):fnv239. PMID:26678554 doi:10.1093/femsle/fnv239
3. ↑ Abdallah J, Mihoub M, Gautier V, Richarme G. The DJ-1 superfamily members YhbO and YajL from Escherichia coli repair proteins from glycation by methylglyoxal and glyoxal. Biochem Biophys Res Commun. 2016 Feb 5;470(2):282-286. PMID:26774339 doi:10.1016/j.bbrc.2016.01.068
4. ↑ Richarme G, Marguet E, Forterre P, Ishino S, Ishino Y. DJ-1 family Maillard deglycases prevent acrylamide formation. Biochem Biophys Res Commun. 2016 Sep 23;478(3):1111-6. PMID:27530919 doi:10.1016/j.bbrc.2016.08.077
5. ↑ Richarme G, Liu C, Mihoub M, Abdallah J, Leger T, Joly N, Liebart JC, Jurkunas UV, Nadal M, Bouloc P, Dairou J, Lamouri A. Guanine glycation repair by DJ-1/Park7 and its bacterial homologs. Science. 2017 Jul 14;357(6347):208-211. PMID:28596309 doi:10.1126/science.aag1095