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1uoj

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(New page: 200px<br /><applet load="1uoj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uoj, resolution 2.40&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1uoj.gif|left|200px]]<br /><applet load="1uoj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1uoj, resolution 2.40&Aring;" />
 
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'''CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN 1 IN THE CALCIUM-FREE STATE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN 1 IN THE CALCIUM-FREE STATE==
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The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in, complex with galactose and calcium was determined at 1.6 A resolution, and, the native protein was solved at 2.4 A resolution. Each monomer adopts a, beta-sandwich fold with ligand binding site at the apex. All galactose, hydroxyl groups, except O1, are involved in a hydrogen bond network with, the protein and O3 and O4 also participate in the co-ordination of the, calcium ion. The stereochemistry of calcium galactose binding is, reminiscent of that observed in some animal C-type lectins. The structure, of the complex provides a framework for future design of anti-bacterial, compounds.
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<StructureSection load='1uoj' size='340' side='right'caption='[[1uoj]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uoj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UOJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uoj OCA], [https://pdbe.org/1uoj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uoj RCSB], [https://www.ebi.ac.uk/pdbsum/1uoj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uoj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 A resolution, and the native protein was solved at 2.4 A resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the co-ordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent of that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.
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==About this Structure==
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Structural basis of calcium and galactose recognition by the lectin PA-IL of Pseudomonas aeruginosa.,Cioci G, Mitchell EP, Gautier C, Wimmerova M, Sudakevitz D, Perez S, Gilboa-Garber N, Imberty A FEBS Lett. 2003 Dec 4;555(2):297-301. PMID:14644431<ref>PMID:14644431</ref>
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1UOJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UOJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis of calcium and galactose recognition by the lectin PA-IL of Pseudomonas aeruginosa., Cioci G, Mitchell EP, Gautier C, Wimmerova M, Sudakevitz D, Perez S, Gilboa-Garber N, Imberty A, FEBS Lett. 2003 Dec 4;555(2):297-301. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14644431 14644431]
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</div>
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[[Category: Pseudomonas aeruginosa]]
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<div class="pdbe-citations 1uoj" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Cioci, G.]]
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[[Category: Gautier, C.]]
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[[Category: Gilboa-Garber, N.]]
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[[Category: Imberty, A.]]
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[[Category: Mitchell, E.]]
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[[Category: Perez, S.]]
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[[Category: Wimmerova, M.]]
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[[Category: SO4]]
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[[Category: galactose binding]]
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[[Category: lectin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:16:36 2007''
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==See Also==
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*[[Galactose-binding lectin|Galactose-binding lectin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Cioci G]]
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[[Category: Gautier C]]
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[[Category: Gilboa-Garber N]]
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[[Category: Imberty A]]
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[[Category: Mitchell E]]
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[[Category: Perez S]]
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[[Category: Wimmerova M]]

Current revision

CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN 1 IN THE CALCIUM-FREE STATE

PDB ID 1uoj

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