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1w52

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Crystal structure of a proteolyzed form of pancreatic lipase related protein 2 from horse

Structural highlights

1w52 is a 1 chain structure with sequence from Equus caballus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.99Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q95KP4_HORSE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Horse pancreatic lipase-related proteins PLRP1 and PLRP2 are produced by the pancreas together with pancreatic lipase (PL). Sequence-comparison analyses reveal that the three proteins possess the same two-domain organization: an N-terminal catalytic domain and a C-terminal domain, which in PL is involved in colipase binding. Nevertheless, despite the high level of sequence identity found, they exhibit distinct enzymatic properties. The intrinsic sensitivity of the peptide bond between Ser245 and Thr246 within the flap region of PLRP2 to proteolytic cleavage probably complicates PLRP2 crystallization since, as shown here, this proteolyzed form of PLRP2 is only crystallized after specific detergent stabilization of this region. This has been performed by the hanging-drop vapour-diffusion method at 291 K and exclusively in the presence of N,N-dimethyldecylamine-beta-oxide (DDAO). However, most crystals (>95%) are highly twinned and diffract poorly (to approximately 7-5 A resolution). Diffraction-quality trigonal crystals have unit-cell parameters a = b = 128.4, c = 85.8 A and belong to space group P3(2)21. A 2.9 A native data set was collected at ESRF on beamline ID14-2 with an R(merge) of 12.7%. Preliminary structural analysis provides a structural basis for the specific roles of DDAO.

Crystallization of a proteolyzed form of the horse pancreatic lipase-related protein 2: structural basis for the specific detergent requirement.,Mancheno JM, Jayne S, Kerfelec B, Chapus C, Crenon I, Hermoso JA Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2107-9. Epub 2004, Oct 20. PMID:15502342^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mancheno JM, Jayne S, Kerfelec B, Chapus C, Crenon I, Hermoso JA. Crystallization of a proteolyzed form of the horse pancreatic lipase-related protein 2: structural basis for the specific detergent requirement. Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2107-9. Epub 2004, Oct 20. PMID:15502342 doi:10.1107/S0907444904024229

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1w52"

@@ Line 3: / Line 3: @@
 <StructureSection load='1w52' size='340' side='right'caption='[[1w52]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1w52]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W52 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1w52]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W52 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.99&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w52 OCA], [http://pdbe.org/1w52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w52 RCSB], [http://www.ebi.ac.uk/pdbsum/1w52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1w52 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w52 OCA], [https://pdbe.org/1w52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w52 RCSB], [https://www.ebi.ac.uk/pdbsum/1w52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w52 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q95KP4_HORSE Q95KP4_HORSE]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 35: @@
 [[Category: Equus caballus]]
 [[Category: Large Structures]]
-[[Category: Chapus, C]]
+[[Category: Chapus C]]
-[[Category: Crenon, I]]
+[[Category: Crenon I]]
-[[Category: Hermoso, J A]]
+[[Category: Hermoso JA]]
-[[Category: Jayne, S]]
+[[Category: Jayne S]]
-[[Category: Kerfelec, B]]
+[[Category: Kerfelec B]]
-[[Category: Mancheno, J M]]
+[[Category: Mancheno JM]]
-[[Category: Cleaved flap]]
-[[Category: Detergent]]
-[[Category: Lipase]]
-[[Category: Pancreatic lipase related protein]]

1w52

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Current revision

Crystal structure of a proteolyzed form of pancreatic lipase related protein 2 from horse

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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