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| | <StructureSection load='2bgl' size='340' side='right'caption='[[2bgl]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='2bgl' size='340' side='right'caption='[[2bgl]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2bgl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mayapple Mayapple]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BGL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2bgl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Podophyllum_peltatum Podophyllum peltatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BGL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAJ:NICOTINAMIDE-ADENINE-DINUCLEOTIDE+(ACIDIC+FORM)'>NAJ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bgk|2bgk]], [[2bgm|2bgm]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAJ:NICOTINAMIDE-ADENINE-DINUCLEOTIDE+(ACIDIC+FORM)'>NAJ</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bgl OCA], [http://pdbe.org/2bgl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bgl RCSB], [http://www.ebi.ac.uk/pdbsum/2bgl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bgl ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bgl OCA], [https://pdbe.org/2bgl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bgl RCSB], [https://www.ebi.ac.uk/pdbsum/2bgl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bgl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SILD_PODPE SILD_PODPE]] Oxidoreductase involved in lignan biosynthesis. Catalyzes the stereospecific conversion of (-)-secoisolariciresinol to (-)-matairesinol via a lactol intermediate.<ref>PMID:11278426</ref> <ref>PMID:16493463</ref> | + | [https://www.uniprot.org/uniprot/SILD_PODPE SILD_PODPE] Oxidoreductase involved in lignan biosynthesis. Catalyzes the stereospecific conversion of (-)-secoisolariciresinol to (-)-matairesinol via a lactol intermediate.<ref>PMID:11278426</ref> <ref>PMID:16493463</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mayapple]] | + | [[Category: Podophyllum peltatum]] |
| - | [[Category: Davin, L B]] | + | [[Category: Davin LB]] |
| - | [[Category: Kang, C]] | + | [[Category: Kang C]] |
| - | [[Category: Lewis, N G]] | + | [[Category: Lewis NG]] |
| - | [[Category: Moinuddin, S G]] | + | [[Category: Moinuddin SG]] |
| - | [[Category: Youn, B]] | + | [[Category: Youn B]] |
| - | [[Category: Dehydrogenase]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Xidoreductase]]
| + | |
| Structural highlights
Function
SILD_PODPE Oxidoreductase involved in lignan biosynthesis. Catalyzes the stereospecific conversion of (-)-secoisolariciresinol to (-)-matairesinol via a lactol intermediate.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
(-)-Matairesinol is a central biosynthetic intermediate to numerous 8-8'-lignans, including the antiviral agent podophyllotoxin in Podophyllum species and its semi-synthetic anticancer derivatives teniposide, etoposide, and Etopophos. It is formed by action of an enantiospecific secoisolariciresinol dehydrogenase, an NAD(H)-dependent oxidoreductase that catalyzes the conversion of (-)-secoisolariciresinol. Matairesinol is also a plant-derived precursor of the cancer-preventative "mammalian" lignan or "phytoestrogen" enterolactone, formed in the gut following ingestion of high fiber dietary foodstuffs, for example. Additionally, secoisolariciresinol dehydrogenase is involved in pathways to important plant defense molecules, such as plicatic acid in the western red cedar (Thuja plicata) heartwood. To understand the molecular and enantiospecific basis of Podophyllum secoisolariciresinol dehydrogenase, crystal structures of the apo-form and binary/ternary complexes were determined at 1.6, 2.8, and 2.0 angstrom resolution, respectively. The enzyme is a homotetramer, consisting of an alpha/beta single domain monomer containing seven parallel beta-strands flanked by eight alpha-helices on both sides. Its overall monomeric structure is similar to that of NAD(H)-dependent short-chain dehydrogenases/reductases, with a conserved Asp47 forming a hydrogen bond with both hydroxyl groups of the adenine ribose of NAD(H), and thus specificity toward NAD(H) instead of NADP(H). The highly conserved catalytic triad (Ser153, Tyr167, and Lys171) is adjacent to both NAD(+) and substrate molecules, where Tyr167 functions as a general base. Following analysis of high resolution structures of the apo-form and two complex forms, the molecular basis for both the enantio-specificity and the reaction mechanism of secoisolariciresinol dehydrogenase is discussed and compared with that of pinoresinol-lariciresinol reductase.
Crystal structures of apo-form and binary/ternary complexes of Podophyllum secoisolariciresinol dehydrogenase, an enzyme involved in formation of health-protecting and plant defense lignans.,Youn B, Moinuddin SG, Davin LB, Lewis NG, Kang C J Biol Chem. 2005 Apr 1;280(13):12917-26. Epub 2005 Jan 13. PMID:15653677[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Xia ZQ, Costa MA, Pelissier HC, Davin LB, Lewis NG. Secoisolariciresinol dehydrogenase purification, cloning, and functional expression. Implications for human health protection. J Biol Chem. 2001 Apr 20;276(16):12614-23. Epub 2001 Jan 18. PMID:11278426 doi:http://dx.doi.org/10.1074/jbc.M008622200
- ↑ Moinuddin SG, Youn B, Bedgar DL, Costa MA, Helms GL, Kang C, Davin LB, Lewis NG. Secoisolariciresinol dehydrogenase: mode of catalysis and stereospecificity of hydride transfer in Podophyllum peltatum. Org Biomol Chem. 2006 Mar 7;4(5):808-16. Epub 2006 Jan 30. PMID:16493463 doi:http://dx.doi.org/10.1039/b516563f
- ↑ Youn B, Moinuddin SG, Davin LB, Lewis NG, Kang C. Crystal structures of apo-form and binary/ternary complexes of Podophyllum secoisolariciresinol dehydrogenase, an enzyme involved in formation of health-protecting and plant defense lignans. J Biol Chem. 2005 Apr 1;280(13):12917-26. Epub 2005 Jan 13. PMID:15653677 doi:10.1074/jbc.M413266200
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