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2c1y

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Structure of PDI-related Chaperone, Wind mutant-Y55K

Structural highlights

2c1y is a 2 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.25Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

WBL_DROME Probable chaperone protein involved in dorsoventral axis patterning in early embryos. Probably acts by folding and targeting pipe (pip) into the Golgi.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structures of the PDI-related protein Wind (with a C-terminal His(6) tag) and the mutants Y53S, Y53F and Y55K have been determined and compared with the wild-type structure with the His(6) tag at the N-terminus. All five structures show the same mode of dimerization, showing that this was not an artefact introduced by the nearby N-terminal His(6) tag and suggesting that this dimer may also be the biologically active form. Although the mutants Y53S and Y55K completely abrogate transport of the protein Pipe (which appears to be the primary function of Wind in the cell), only subtle differences can be seen in the putative Pipe-binding region. The Pipe binding in the active forms appears to involve hydrophobic interactions between aromatic systems, whereas the inactive mutants may be able to bind more strongly with the help of hydrogen bonds, which could disturb the delicate equilibrium required for effective Pipe transport.

Structural elucidation of the PDI-related chaperone Wind with the help of mutants.,Sevvana M, Biadene M, Ma Q, Guo C, Soling HD, Sheldrick GM, Ferrari DM Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):589-94. Epub 2006, May 12. PMID:16699185^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nilson LA, Schupbach T. Localized requirements for windbeutel and pipe reveal a dorsoventral prepattern within the follicular epithelium of the Drosophila ovary. Cell. 1998 Apr 17;93(2):253-62. PMID:9568717
↑ Sen J, Goltz JS, Konsolaki M, Schupbach T, Stein D. Windbeutel is required for function and correct subcellular localization of the Drosophila patterning protein Pipe. Development. 2000 Dec;127(24):5541-50. PMID:11076773
↑ Sevvana M, Biadene M, Ma Q, Guo C, Soling HD, Sheldrick GM, Ferrari DM. Structural elucidation of the PDI-related chaperone Wind with the help of mutants. Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):589-94. Epub 2006, May 12. PMID:16699185 doi:10.1107/S0907444906010456

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c1y"

@@ Line 3: / Line 3: @@
 <StructureSection load='2c1y' size='340' side='right'caption='[[2c1y]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2c1y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C1Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2c1y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C1Y FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ovn|1ovn]], [[2c0e|2c0e]], [[2c0f|2c0f]], [[2c0g|2c0g]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c1y OCA], [http://pdbe.org/2c1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c1y RCSB], [http://www.ebi.ac.uk/pdbsum/2c1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2c1y ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c1y OCA], [https://pdbe.org/2c1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c1y RCSB], [https://www.ebi.ac.uk/pdbsum/2c1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c1y ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/WBL_DROME WBL_DROME] Probable chaperone protein involved in dorsoventral axis patterning in early embryos. Probably acts by folding and targeting pipe (pip) into the Golgi.<ref>PMID:9568717</ref> <ref>PMID:11076773</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: Barnewitz, K]]
+[[Category: Barnewitz K]]
-[[Category: Ferrari, D M]]
+[[Category: Ferrari DM]]
-[[Category: Guo, C]]
+[[Category: Guo C]]
-[[Category: Ma, Q]]
+[[Category: Ma Q]]
-[[Category: Sevvana, M]]
+[[Category: Sevvana M]]
-[[Category: Sheldrick, G M]]
+[[Category: Sheldrick GM]]
-[[Category: Soling, H D]]
+[[Category: Soling H-D]]
-[[Category: Chaperone]]
-[[Category: Developmental protein]]
-[[Category: Dorsal-ventral patterning]]
-[[Category: Endoplasmic reticulum]]
-[[Category: Pdi]]
-[[Category: Pdi-dbeta]]
-[[Category: Pipe]]
-[[Category: Protein disulfide isomerase]]
-[[Category: Wind]]
-[[Category: Wind mutant]]
-[[Category: Windbeutel]]

2c1y

From Proteopedia

Current revision

Structure of PDI-related Chaperone, Wind mutant-Y55K

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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