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2cfa

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{{Seed}}
 
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[[Image:2cfa.png|left|200px]]
 
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==Structure of viral flavin-dependant thymidylate synthase ThyX==
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The line below this paragraph, containing "STRUCTURE_2cfa", creates the "Structure Box" on the page.
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<StructureSection load='2cfa' size='340' side='right'caption='[[2cfa]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CFA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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{{STRUCTURE_2cfa| PDB=2cfa | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [https://pdbe.org/2cfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [https://www.ebi.ac.uk/pdbsum/2cfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cfa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/THYX_PBCV1 THYX_PBCV1] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cfa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cfa ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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By using biochemical and structural analyses, we have investigated the catalytic mechanism of the recently discovered flavin-dependent thymidylate synthase ThyX from Paramecium bursaria chlorella virus-1 (PBCV-1). Site-directed mutagenesis experiments have identified several residues implicated in either NADPH oxidation or deprotonation activity of PBCV-1 ThyX. Chemical modification by diethyl pyrocarbonate and mass spectroscopic analyses identified a histidine residue (His53) crucial for NADPH oxidation and located in the vicinity of the redox active N-5 atom of the FAD ring system. Moreover, we observed that the conformation of active site key residues of PBCV-1 ThyX differs from earlier reported ThyX structures, suggesting structural changes during catalysis. Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate.
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===STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX===
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Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX.,Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U J Biol Chem. 2006 Aug 18;281(33):24048-57. Epub 2006 May 17. PMID:16707489<ref>PMID:16707489</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2cfa" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16707489}}, adds the Publication Abstract to the page
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*[[Thymidylate synthase 3D structures|Thymidylate synthase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16707489 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16707489}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2CFA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA].
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[[Category: Paramecium bursaria Chlorella virus 1]]
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[[Category: Bernauer J]]
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==Reference==
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[[Category: Decottignies P]]
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Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX., Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U, J Biol Chem. 2006 Aug 18;281(33):24048-57. Epub 2006 May 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16707489 16707489]
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[[Category: Graille M]]
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[[Category: Paramecium bursaria chlorella virus 1]]
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[[Category: Graziani S]]
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[[Category: Protein complex]]
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[[Category: Liebl U]]
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[[Category: Bernauer, J.]]
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[[Category: Marchand C]]
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[[Category: Decottignies, P.]]
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[[Category: Myllykallio H]]
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[[Category: Graille, M.]]
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[[Category: Skouloubris S]]
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[[Category: Graziani, S.]]
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[[Category: Zhou C-Z]]
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[[Category: Liebl, U.]]
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[[Category: Van Tilbeurgh H]]
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[[Category: Marchand, C.]]
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[[Category: Myllykallio, H.]]
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[[Category: Skouloubris, S.]]
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[[Category: Tilbeurgh, H Van.]]
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[[Category: Zhou, C Z.]]
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[[Category: Fdt]]
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[[Category: Flavin dependent thymidylate synthase fad]]
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[[Category: Flavoprotein]]
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[[Category: Methyltransferase]]
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[[Category: Nucleotide biosynthesis]]
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[[Category: Paramecium bursaria chlorella virus-1]]
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[[Category: Thyx]]
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[[Category: Transferase]]
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[[Category: Tscp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:27:48 2008''
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Current revision

Structure of viral flavin-dependant thymidylate synthase ThyX

PDB ID 2cfa

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