2iv3

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[[Image:2iv3.gif|left|200px]]<br /><applet load="2iv3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2iv3, resolution 2.30&Aring;" />
 
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'''CRYSTAL STRUCTURE OF AVIGT4, A GLYCOSYLTRANSFERASE INVOLVED IN AVILAMYCIN A BIOSYNTHESIS'''<br />
 
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==Overview==
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==Crystal structure of AviGT4, a glycosyltransferase involved in Avilamycin A biosynthesis==
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Glycosyltransferases (GTs) catalyze the synthesis of the myriad, glycoconjugates that are central to life. One of the largest families is, GT4, which contains several enzymes of therapeutic significance, exemplified by WaaG and AviGT4. WaaG catalyses a key step in, lipopolysaccharide synthesis, while AviGT4, produced by Streptomyces, viridochromogenes, contributes to the synthesis of the antibiotic, avilamycin A. Here we present the crystal structure of both WaaG and, AviGT4. The two enzymes contain two "Rossmann-like" (beta/alpha/beta), domains characteristic of the GT-B fold. Both recognition of the donor, substrate and the catalytic machinery is similar to other retaining GTs, that display the GT-B fold. Structural information is discussed with, respect to the evolution of GTs and the therapeutic significance of the, two enzymes.
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<StructureSection load='2iv3' size='340' side='right'caption='[[2iv3]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2iv3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_viridochromogenes Streptomyces viridochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IV3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iv3 OCA], [https://pdbe.org/2iv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iv3 RCSB], [https://www.ebi.ac.uk/pdbsum/2iv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iv3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q93KV2_STRVR Q93KV2_STRVR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/2iv3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iv3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glycosyltransferases (GTs) catalyze the synthesis of the myriad glycoconjugates that are central to life. One of the largest families is GT4, which contains several enzymes of therapeutic significance, exemplified by WaaG and AviGT4. WaaG catalyses a key step in lipopolysaccharide synthesis, while AviGT4, produced by Streptomyces viridochromogenes, contributes to the synthesis of the antibiotic avilamycin A. Here we present the crystal structure of both WaaG and AviGT4. The two enzymes contain two "Rossmann-like" (beta/alpha/beta) domains characteristic of the GT-B fold. Both recognition of the donor substrate and the catalytic machinery is similar to other retaining GTs that display the GT-B fold. Structural information is discussed with respect to the evolution of GTs and the therapeutic significance of the two enzymes.
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==About this Structure==
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Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4.,Martinez-Fleites C, Proctor M, Roberts S, Bolam DN, Gilbert HJ, Davies GJ Chem Biol. 2006 Nov;13(11):1143-52. PMID:17113996<ref>PMID:17113996</ref>
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2IV3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_viridochromogenes Streptomyces viridochromogenes] with UDP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol Binding Site For Chain D'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IV3 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4., Martinez-Fleites C, Proctor M, Roberts S, Bolam DN, Gilbert HJ, Davies GJ, Chem Biol. 2006 Nov;13(11):1143-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17113996 17113996]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2iv3" style="background-color:#fffaf0;"></div>
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[[Category: Streptomyces viridochromogenes]]
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[[Category: Bolam, D.N.]]
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[[Category: Davies, G.J.]]
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[[Category: Gilbert, H.J.]]
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[[Category: Martinez-Fleites, C.]]
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[[Category: Proctor, M.]]
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[[Category: Roberts, S.]]
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[[Category: GOL]]
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[[Category: UDP]]
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[[Category: antibiotics]]
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[[Category: avilamycin a]]
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[[Category: family gt-4]]
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[[Category: glycosyltransferase]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:35:57 2007''
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==See Also==
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptomyces viridochromogenes]]
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[[Category: Bolam DN]]
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[[Category: Davies GJ]]
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[[Category: Gilbert HJ]]
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[[Category: Martinez-Fleites C]]
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[[Category: Proctor M]]
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[[Category: Roberts S]]

Current revision

Crystal structure of AviGT4, a glycosyltransferase involved in Avilamycin A biosynthesis

PDB ID 2iv3

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