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| | <StructureSection load='2j5l' size='340' side='right'caption='[[2j5l]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='2j5l' size='340' side='right'caption='[[2j5l]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2j5l]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J5L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2j5l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J5L FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j5l OCA], [http://pdbe.org/2j5l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j5l RCSB], [http://www.ebi.ac.uk/pdbsum/2j5l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j5l ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j5l OCA], [https://pdbe.org/2j5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j5l RCSB], [https://www.ebi.ac.uk/pdbsum/2j5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j5l ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9BIM8_PLAFA Q9BIM8_PLAFA] Involved in parasite invasion of erythrocytes.[ARBA:ARBA00002326] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Mus musculus]] | | [[Category: Mus musculus]] |
| - | [[Category: Plafa]] | + | [[Category: Plasmodium falciparum]] |
| - | [[Category: Bentley, G A]] | + | [[Category: Bentley GA]] |
| - | [[Category: Faure, G]] | + | [[Category: Faure G]] |
| - | [[Category: Igonet, S]] | + | [[Category: Igonet S]] |
| - | [[Category: Kocken, C H.M]] | + | [[Category: Kocken CHM]] |
| - | [[Category: Normand, B Vulliez-Le]]
| + | [[Category: Riottot MM]] |
| - | [[Category: Riottot, M M]] | + | [[Category: Thomas AW]] |
| - | [[Category: Thomas, A W]] | + | [[Category: Vulliez-Le Normand B]] |
| - | [[Category: Antibody cross-reactivity]] | + | |
| - | [[Category: Apical membrane antigen 1]]
| + | |
| - | [[Category: Hypothetical protein]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunoglobulin c region]]
| + | |
| - | [[Category: Immunoglobulin domain]]
| + | |
| - | [[Category: Malaria vaccine candidate]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Merozoite]]
| + | |
| - | [[Category: Transmembrane]]
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| Structural highlights
Function
Q9BIM8_PLAFA Involved in parasite invasion of erythrocytes.[ARBA:ARBA00002326]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Apical membrane antigen 1 (AMA1) has an important, but as yet uncharacterised, role in host cell invasion by the malaria parasite, Plasmodium. The protein, which is quite conserved between Plasmodium species, comprises an ectoplasmic region, a single transmembrane segment and a small cytoplasmic domain. The ectoplasmic region, which can induce protective immunity in animal models of human malaria, is a leading vaccine candidate that has entered clinical trials. The monoclonal antibody F8.12.19, raised against the recombinant ectoplasmic region of AMA1 from Plasmodium vivax, cross-reacts with homologues from Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium berghei and Plasmodium falciparum, as shown by immunofluorescence assays on mature schizonts. The binding of F8.12.19 to recombinant AMA1 from both P. vivax and P. falciparum was measured by surface plasmon resonance, revealing an apparent affinity constant that is about 100-fold weaker for the cross-reacting antigen when compared to the cognate antigen. Crystal structure analysis of Fab F8.12.19 complexed to AMA1 from P. vivax and P. falciparum shows that the monoclonal antibody recognises a discontinuous epitope located on domain III of the ectoplasmic region, the major component being a loop containing a cystine knot. The structures provide a basis for understanding the cross-reactivity. Antibody contacts are made mainly to main-chain and invariant side-chain atoms of AMA1; contact antigen residues that differ in sequence are located at the periphery of the antigen-binding site and can be accommodated at the interface between the two components of the complex. The implications for AMA1 vaccine development are discussed.
Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation.,Igonet S, Vulliez-Le Normand B, Faure G, Riottot MM, Kocken CH, Thomas AW, Bentley GA J Mol Biol. 2007 Mar 9;366(5):1523-37. Epub 2006 Dec 16. PMID:17229439[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Igonet S, Vulliez-Le Normand B, Faure G, Riottot MM, Kocken CH, Thomas AW, Bentley GA. Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation. J Mol Biol. 2007 Mar 9;366(5):1523-37. Epub 2006 Dec 16. PMID:17229439 doi:http://dx.doi.org/10.1016/j.jmb.2006.12.028
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