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2j5l

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Current revision

Structure of a Plasmodium falciparum apical membrane antigen 1-Fab F8. 12.19 complex

Structural highlights

2j5l is a 3 chain structure with sequence from Mus musculus and Plasmodium falciparum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9BIM8_PLAFA Involved in parasite invasion of erythrocytes.[ARBA:ARBA00002326]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Apical membrane antigen 1 (AMA1) has an important, but as yet uncharacterised, role in host cell invasion by the malaria parasite, Plasmodium. The protein, which is quite conserved between Plasmodium species, comprises an ectoplasmic region, a single transmembrane segment and a small cytoplasmic domain. The ectoplasmic region, which can induce protective immunity in animal models of human malaria, is a leading vaccine candidate that has entered clinical trials. The monoclonal antibody F8.12.19, raised against the recombinant ectoplasmic region of AMA1 from Plasmodium vivax, cross-reacts with homologues from Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium berghei and Plasmodium falciparum, as shown by immunofluorescence assays on mature schizonts. The binding of F8.12.19 to recombinant AMA1 from both P. vivax and P. falciparum was measured by surface plasmon resonance, revealing an apparent affinity constant that is about 100-fold weaker for the cross-reacting antigen when compared to the cognate antigen. Crystal structure analysis of Fab F8.12.19 complexed to AMA1 from P. vivax and P. falciparum shows that the monoclonal antibody recognises a discontinuous epitope located on domain III of the ectoplasmic region, the major component being a loop containing a cystine knot. The structures provide a basis for understanding the cross-reactivity. Antibody contacts are made mainly to main-chain and invariant side-chain atoms of AMA1; contact antigen residues that differ in sequence are located at the periphery of the antigen-binding site and can be accommodated at the interface between the two components of the complex. The implications for AMA1 vaccine development are discussed.

Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation.,Igonet S, Vulliez-Le Normand B, Faure G, Riottot MM, Kocken CH, Thomas AW, Bentley GA J Mol Biol. 2007 Mar 9;366(5):1523-37. Epub 2006 Dec 16. PMID:17229439^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Igonet S, Vulliez-Le Normand B, Faure G, Riottot MM, Kocken CH, Thomas AW, Bentley GA. Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation. J Mol Biol. 2007 Mar 9;366(5):1523-37. Epub 2006 Dec 16. PMID:17229439 doi:http://dx.doi.org/10.1016/j.jmb.2006.12.028

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j5l"

@@ Line 3: / Line 3: @@
 <StructureSection load='2j5l' size='340' side='right'caption='[[2j5l]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2j5l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J5L FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2j5l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J5L FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j5l OCA], [https://pdbe.org/2j5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j5l RCSB], [https://www.ebi.ac.uk/pdbsum/2j5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j5l ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9BIM8_PLAFA Q9BIM8_PLAFA] Involved in parasite invasion of erythrocytes.[ARBA:ARBA00002326]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 38: @@
 [[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Plafa]]
+[[Category: Plasmodium falciparum]]
-[[Category: Bentley, G A]]
+[[Category: Bentley GA]]
-[[Category: Faure, G]]
+[[Category: Faure G]]
-[[Category: Igonet, S]]
+[[Category: Igonet S]]
-[[Category: Kocken, C H.M]]
+[[Category: Kocken CHM]]
-[[Category: Normand, B Vulliez-Le]]
+[[Category: Riottot MM]]
-[[Category: Riottot, M M]]
+[[Category: Thomas AW]]
-[[Category: Thomas, A W]]
+[[Category: Vulliez-Le Normand B]]
-[[Category: Antibody cross-reactivity]]
-[[Category: Apical membrane antigen 1]]
-[[Category: Hypothetical protein]]
-[[Category: Immune system]]
-[[Category: Immunoglobulin c region]]
-[[Category: Immunoglobulin domain]]
-[[Category: Malaria vaccine candidate]]
-[[Category: Membrane]]
-[[Category: Merozoite]]
-[[Category: Transmembrane]]

2j5l

From Proteopedia

Current revision

Structure of a Plasmodium falciparum apical membrane antigen 1-Fab F8. 12.19 complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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