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2j7k

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[[Image:2j7k.jpg|left|200px]]<br /><applet load="2j7k" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2j7k, resolution 2.90&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE T84A MUTANT EF-G:GDPCP COMPLEX'''<br />
 
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==About this Structure==
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==Crystal structure of the T84A mutant EF-G:GDPCP complex==
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2J7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GCP:'>GCP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA].
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<StructureSection load='2j7k' size='340' side='right'caption='[[2j7k]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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[[Category: Protein-synthesizing GTPase]]
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== Structural highlights ==
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[[Category: Single protein]]
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<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J7K FirstGlance]. <br>
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[[Category: Thermus thermophilus]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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[[Category: Hansson, S.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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[[Category: Logan, D.T.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [https://pdbe.org/2j7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [https://www.ebi.ac.uk/pdbsum/2j7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7k ProSAT]</span></td></tr>
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[[Category: GCP]]
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</table>
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[[Category: MG]]
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== Function ==
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[[Category: elongation factor]]
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[https://www.uniprot.org/uniprot/EFG_THETH EFG_THETH] Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
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[[Category: gtp-binding]]
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== Evolutionary Conservation ==
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[[Category: mutation]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: nucleotide- binding]]
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Check<jmol>
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[[Category: nucleotide-binding]]
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<jmolCheckbox>
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[[Category: p-loop]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/2j7k_consurf.spt"</scriptWhenChecked>
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[[Category: protein biosynthesis]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: thr84ala]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: translation]]
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j7k ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:42:32 2008''
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==See Also==
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Hansson S]]
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[[Category: Logan DT]]

Current revision

Crystal structure of the T84A mutant EF-G:GDPCP complex

PDB ID 2j7k

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