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2jjz

From Proteopedia

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[[Image:2jjz.png|left|200px]]
 
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==Crystal Structure of Human Iba2, orthorhombic crystal form==
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The line below this paragraph, containing "STRUCTURE_2jjz", creates the "Structure Box" on the page.
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<StructureSection load='2jjz' size='340' side='right'caption='[[2jjz]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jjz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JJZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2jjz| PDB=2jjz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jjz OCA], [https://pdbe.org/2jjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jjz RCSB], [https://www.ebi.ac.uk/pdbsum/2jjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jjz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AIF1L_HUMAN AIF1L_HUMAN] Actin-binding protein that promotes actin bundling. May neither bind calcium nor depend on calcium for function.<ref>PMID:18699778</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/2jjz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jjz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Iba2 is a homolog of ionized calcium-binding adapter molecule 1 (Iba1), a 17-kDa protein that binds and cross-links filamentous actin (F-actin) and localizes to membrane ruffles and phagocytic cups. Here, we present the crystal structure of human Iba2 and its homodimerization properties, F-actin cross-linking activity, cellular localization and recruitment upon bacterial invasion in comparison with Iba1. The Iba2 structure comprises two central EF-hand motifs lacking bound Ca2+. Iba2 crystallized as a homodimer stabilized by a disulfide bridge and zinc ions. Analytical ultracentrifugation revealed a different mode of dimerization under reducing conditions that was independent of Ca2+. Furthermore, no binding of Ca2+ up to 0.1 mM was detected by equilibrium dialysis. Correspondingly, Iba EF-hand motifs lack residues essential for strong Ca2+ coordination. Sedimentation experiments and microscopy detected pronounced, indistinguishable F-actin binding and cross-linking activity of Iba1 and Iba2 with induction of F-actin bundles. Fluorescent Iba fusion proteins were expressed in HeLa cells and co-localized with F-actin. Iba1 was recruited into cellular projections to a larger extent than Iba2. Additionally, we studied Iba recruitment in a Shigella invasion model that induces cytoskeletal rearrangements. Both proteins were recruited into the bacterial invasion zone and Iba1 was again concentrated slightly higher in the cellular extensions.
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===CRYSTAL STRUCTURE OF HUMAN IBA2, ORTHORHOMBIC CRYSTAL FORM===
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Structural and functional characterization of human Iba proteins.,Schulze JO, Quedenau C, Roske Y, Adam T, Schuler H, Behlke J, Turnbull AP, Sievert V, Scheich C, Mueller U, Heinemann U, Bussow K FEBS J. 2008 Sep;275(18):4627-40. Epub 2008 Aug 11. PMID:18699778<ref>PMID:18699778</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18699778}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2jjz" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18699778 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18699778}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2jjz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJZ OCA].
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==Reference==
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<ref group="xtra">PMID:018699778</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Buessow, K.]]
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[[Category: Large Structures]]
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[[Category: Heinemann, U.]]
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[[Category: Buessow K]]
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[[Category: Mueller, U.]]
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[[Category: Heinemann U]]
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[[Category: Quedenau, C.]]
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[[Category: Mueller U]]
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[[Category: Roske, Y.]]
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[[Category: Quedenau C]]
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[[Category: Schulze, J O.]]
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[[Category: Roske Y]]
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[[Category: Turnbull, A.]]
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[[Category: Schulze JO]]
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[[Category: Actin crosslinking]]
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[[Category: Turnbull A]]
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[[Category: Calcium binding]]
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[[Category: Ef-hand]]
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[[Category: Ionized calcium binding adapter molecule 2]]
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[[Category: Metal-binding protein]]
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Current revision

Crystal Structure of Human Iba2, orthorhombic crystal form

PDB ID 2jjz

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