2v0c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:00, 13 December 2023) (edit) (undo)
 
(22 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2v0c.gif|left|200px]]<br />
 
-
<applet load="2v0c" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2v0c, resolution 1.85&Aring;" />
 
-
'''LEUCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH A SULPHAMOYL ANALOGUE OF LEUCYL-ADENYLATE IN THE SYNTHETIC SITE AND AN ADDUCT OF AMP WITH 5-FLUORO-1,3-DIHYDRO-1-HYDROXY-2,1-BENZOXABOROLE (AN2690) IN THE EDITING SITE'''<br />
 
-
==Overview==
+
==LEUCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH A SULPHAMOYL ANALOGUE OF LEUCYL-ADENYLATE In the synthetic site and an adduct of AMP with 5-Fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole (AN2690) in the editing site==
-
Aminoacyl-transfer RNA (tRNA) synthetases, which catalyze the attachment, of the correct amino acid to its corresponding tRNA during translation of, the genetic code, are proven antimicrobial drug targets. We show that the, broad-spectrum antifungal 5-fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole, (AN2690), in development for the treatment of onychomycosis, inhibits, yeast cytoplasmic leucyl-tRNA synthetase by formation of a stable, tRNA(Leu)-AN2690 adduct in the editing site of the enzyme. Adduct, formation is mediated through the boron atom of AN2690 and the 2'- and, 3'-oxygen atoms of tRNA's3'-terminal adenosine. The trapping of, enzyme-bound tRNA(Leu) in the editing site prevents catalytic turnover, thus inhibiting synthesis of leucyl-tRNA(Leu) and consequentially blocking, protein ... [[http://ispc.weizmann.ac.il/pmbin/getpm?17588934 (full description)]]
+
<StructureSection load='2v0c' size='340' side='right'caption='[[2v0c]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2v0c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V0C FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANZ:[(6-AMINO-9H-PURIN-9-YL)-[5-FLUORO-1,3-DIHYDRO-1-HYDROXY-2,1-BENZOXABOROLE]-4YL]METHYL+DIHYDROGEN+PHOSPHATE'>ANZ</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=LMS:[(2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXYTETRAHYDRO-2-FURANYL]METHYL+SULFAMATE'>LMS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v0c OCA], [https://pdbe.org/2v0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v0c RCSB], [https://www.ebi.ac.uk/pdbsum/2v0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v0c ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q7SIE4_THETH Q7SIE4_THETH]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v0/2v0c_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v0c ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Aminoacyl-transfer RNA (tRNA) synthetases, which catalyze the attachment of the correct amino acid to its corresponding tRNA during translation of the genetic code, are proven antimicrobial drug targets. We show that the broad-spectrum antifungal 5-fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole (AN2690), in development for the treatment of onychomycosis, inhibits yeast cytoplasmic leucyl-tRNA synthetase by formation of a stable tRNA(Leu)-AN2690 adduct in the editing site of the enzyme. Adduct formation is mediated through the boron atom of AN2690 and the 2'- and 3'-oxygen atoms of tRNA's3'-terminal adenosine. The trapping of enzyme-bound tRNA(Leu) in the editing site prevents catalytic turnover, thus inhibiting synthesis of leucyl-tRNA(Leu) and consequentially blocking protein synthesis. This result establishes the editing site as a bona fide target for aminoacyl-tRNA synthetase inhibitors.
-
==About this Structure==
+
An antifungal agent inhibits an aminoacyl-tRNA synthetase by trapping tRNA in the editing site.,Rock FL, Mao W, Yaremchuk A, Tukalo M, Crepin T, Zhou H, Zhang YK, Hernandez V, Akama T, Baker SJ, Plattner JJ, Shapiro L, Martinis SA, Benkovic SJ, Cusack S, Alley MR Science. 2007 Jun 22;316(5832):1759-61. PMID:17588934<ref>PMID:17588934</ref>
-
2V0C is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]] with ZN, SO4, LEU and ANZ as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V0C OCA]].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
An antifungal agent inhibits an aminoacyl-tRNA synthetase by trapping tRNA in the editing site., Rock FL, Mao W, Yaremchuk A, Tukalo M, Crepin T, Zhou H, Zhang YK, Hernandez V, Akama T, Baker SJ, Plattner JJ, Shapiro L, Martinis SA, Benkovic SJ, Cusack S, Alley MR, Science. 2007 Jun 22;316(5832):1759-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17588934 17588934]
+
</div>
-
[[Category: Single protein]]
+
<div class="pdbe-citations 2v0c" style="background-color:#fffaf0;"></div>
-
[[Category: Thermus thermophilus]]
+
-
[[Category: Akama, T.]]
+
-
[[Category: Alley, M.R.K.]]
+
-
[[Category: Baker, S.]]
+
-
[[Category: Benkovic, S.J.]]
+
-
[[Category: Crepin, T.]]
+
-
[[Category: Cusack, S.]]
+
-
[[Category: Hernandez, V.]]
+
-
[[Category: Mao, W.]]
+
-
[[Category: Martinis, S.A.]]
+
-
[[Category: Plattner, J.]]
+
-
[[Category: Rock, F.]]
+
-
[[Category: Shapiro, L.]]
+
-
[[Category: Tukalo, M.]]
+
-
[[Category: Yaremchuk, A.]]
+
-
[[Category: Zhang, Y.]]
+
-
[[Category: Zhou, H.]]
+
-
[[Category: ANZ]]
+
-
[[Category: LEU]]
+
-
[[Category: SO4]]
+
-
[[Category: ZN]]
+
-
[[Category: aminoacyl-trna synthetase]]
+
-
[[Category: atp + l-leucine + trna (leu) gives amp + ppi + l-leucyl-trna(leu)]]
+
-
[[Category: atp-binding]]
+
-
[[Category: clss i aminoacyl-trna synthetase]]
+
-
[[Category: ligase]]
+
-
[[Category: metal-binding]]
+
-
[[Category: nucleotide-binding]]
+
-
[[Category: protein biosynthesis]]
+
-
[[Category: zinc]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:41:42 2007''
+
==See Also==
 +
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Thermus thermophilus]]
 +
[[Category: Akama T]]
 +
[[Category: Alley MRK]]
 +
[[Category: Baker S]]
 +
[[Category: Benkovic SJ]]
 +
[[Category: Crepin T]]
 +
[[Category: Cusack S]]
 +
[[Category: Hernandez V]]
 +
[[Category: Mao W]]
 +
[[Category: Martinis SA]]
 +
[[Category: Plattner J]]
 +
[[Category: Rock F]]
 +
[[Category: Shapiro L]]
 +
[[Category: Tukalo M]]
 +
[[Category: Yaremchuk A]]
 +
[[Category: Zhang Y]]
 +
[[Category: Zhou H]]

Current revision

LEUCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH A SULPHAMOYL ANALOGUE OF LEUCYL-ADENYLATE In the synthetic site and an adduct of AMP with 5-Fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole (AN2690) in the editing site

PDB ID 2v0c

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v0c"
Personal tools