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2v4e

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[[Image:2v4e.png|left|200px]]
 
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{{STRUCTURE_2v4e| PDB=2v4e | SCENE= }}
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==A non-cytotoxic DsRed variant for whole-cell labeling==
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<StructureSection load='2v4e' size='340' side='right'caption='[[2v4e]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2v4e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V4E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v4e OCA], [https://pdbe.org/2v4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v4e RCSB], [https://www.ebi.ac.uk/pdbsum/2v4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v4e ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v4/2v4e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v4e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
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===A NON-CYTOTOXIC DSRED VARIANT FOR WHOLE-CELL LABELING===
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A noncytotoxic DsRed variant for whole-cell labeling.,Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer HE, Keenan RJ, Glick BS Nat Methods. 2008 Nov;5(11):955-7. Epub 2008 Oct 26. PMID:18953349<ref>PMID:18953349</ref>
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{{ABSTRACT_PUBMED_18953349}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2v4e" style="background-color:#fffaf0;"></div>
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[[2v4e]] is a 8 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V4E OCA].
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==See Also==
==See Also==
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*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
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== References ==
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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<references/>
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*[[Sandbox104|Sandbox104]]
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__TOC__
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*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
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</StructureSection>
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[[Category: Discosoma sp]]
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==Reference==
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[[Category: Large Structures]]
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<ref group="xtra">PMID:018953349</ref><references group="xtra"/>
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[[Category: Berman A]]
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[[Category: Discosoma sp.]]
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[[Category: Bhattacharyya D]]
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[[Category: Berman, A.]]
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[[Category: Broxmeyer HE]]
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[[Category: Bhattacharyya, D.]]
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[[Category: Glick BS]]
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[[Category: Broxmeyer, H E.]]
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[[Category: Keenan RJ]]
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[[Category: Glick, B S.]]
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[[Category: Strack RL]]
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[[Category: Keenan, R J.]]
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[[Category: Strongin DE]]
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[[Category: Strack, R L.]]
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[[Category: Tao W]]
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[[Category: Strongin, D E.]]
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[[Category: Tao, W.]]
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[[Category: Chromophore]]
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[[Category: Dsred]]
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[[Category: Fluorescent protein]]
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[[Category: Luminescence]]
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[[Category: Photoprotein]]
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Current revision

A non-cytotoxic DsRed variant for whole-cell labeling

PDB ID 2v4e

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