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2v8n

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[[Image:2v8n.jpg|left|200px]]<br /><applet load="2v8n" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2v8n, resolution 3.60&Aring;" />
 
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'''WILD-TYPE STRUCTURE OF LACTOSE PERMEASE'''<br />
 
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==Overview==
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==Wild-type Structure of Lactose Permease==
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Here we describe an x-ray structure of wild-type lactose permease (LacY), from Escherichia coli determined by manipulating phospholipid content, during crystallization. The structure exhibits the same global fold as the, previous x-ray structures of a mutant that binds sugar but cannot catalyze, translocation across the membrane. LacY is organized into two six-helix, bundles with twofold pseudosymmetry separated by a large interior, hydrophilic cavity open only to the cytoplasmic side and containing the, side chains important for sugar and H(+) binding. To initiate transport, binding of sugar and/or an H(+) electrochemical gradient increases the, probability of opening on the periplasmic side. Because the inward-facing, conformation represents the lowest free-energy state, the rate-limiting, step for transport may be the conformational change leading to the, outward-facing conformation.
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<StructureSection load='2v8n' size='340' side='right'caption='[[2v8n]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2v8n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V8N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v8n OCA], [https://pdbe.org/2v8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v8n RCSB], [https://www.ebi.ac.uk/pdbsum/2v8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v8n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v8/2v8n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v8n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Here we describe an x-ray structure of wild-type lactose permease (LacY) from Escherichia coli determined by manipulating phospholipid content during crystallization. The structure exhibits the same global fold as the previous x-ray structures of a mutant that binds sugar but cannot catalyze translocation across the membrane. LacY is organized into two six-helix bundles with twofold pseudosymmetry separated by a large interior hydrophilic cavity open only to the cytoplasmic side and containing the side chains important for sugar and H(+) binding. To initiate transport, binding of sugar and/or an H(+) electrochemical gradient increases the probability of opening on the periplasmic side. Because the inward-facing conformation represents the lowest free-energy state, the rate-limiting step for transport may be the conformational change leading to the outward-facing conformation.
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==About this Structure==
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Structural determination of wild-type lactose permease.,Guan L, Mirza O, Verner G, Iwata S, Kaback HR Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15294-8. Epub 2007 Sep 19. PMID:17881559<ref>PMID:17881559</ref>
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2V8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V8N OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural determination of wild-type lactose permease., Guan L, Mirza O, Verner G, Iwata S, Kaback HR, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15294-8. Epub 2007 Sep 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17881559 17881559]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 2v8n" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Guan, L.]]
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[[Category: Iwata, S.]]
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[[Category: Kaback, H.R.]]
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[[Category: Mirza, O.]]
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[[Category: Verner, G.]]
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[[Category: formylation]]
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[[Category: inner membrane]]
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[[Category: membrane]]
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[[Category: sugar transport]]
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[[Category: symport]]
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[[Category: transmembrane]]
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[[Category: transport]]
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[[Category: transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:58:51 2008''
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==See Also==
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*[[Lactose Permease|Lactose Permease]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Guan L]]
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[[Category: Iwata S]]
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[[Category: Kaback HR]]
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[[Category: Mirza O]]
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[[Category: Verner G]]

Current revision

Wild-type Structure of Lactose Permease

PDB ID 2v8n

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