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2va1

From Proteopedia

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Current revision

Crystal structure of UMP kinase from Ureaplasma parvum

Structural highlights

2va1 is a 6 chain structure with sequence from Ureaplasma parvum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PYRH_UREPA Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor. Is also able to phosphorylate dUMP.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of uridine monophosphate kinase (UMP kinase, UMPK) from the opportunistic pathogen Ureaplasma parvum was determined and showed similar three-dimensional fold as other bacterial and archaeal UMPKs that all belong to the amino acid kinase family. Recombinant UpUMPK exhibited Michaelis-Menten kinetics with UMP, with K(m) and V(max) values of 214 +/- 4 microm and 262 +/- 24 micromol.min(-1).mg(-1), respectively, but with ATP as variable substrate the kinetic analysis showed positive cooperativity, with an n value of 1.5 +/- 0.1. The end-product UTP was a competitive inhibitor against UMP and a noncompetitive inhibitor towards ATP. Unlike UMPKs from other bacteria, which are activated by GTP, GTP had no detectable effect on UpUMPK activity. An attempt to create a GTP-activated enzyme was made using site-directed mutagenesis. The mutant enzyme F133N (F133 corresponds to the residue in Escherichia coli that is involved in GTP activation), with F133A as a control, were expressed, purified and characterized. Both enzymes exhibited negative cooperativity with UMP, and GTP had no effect on enzyme activity, demonstrating that F133 is involved in subunit interactions but apparently not in GTP activation. The physiological role of UpUMPK in bacterial nucleic acid synthesis and its potential as target for development of antimicrobial agents are discussed.

Structural and functional investigations of Ureaplasma parvum UMP kinase--a potential antibacterial drug target.,Egeblad-Welin L, Welin M, Wang L, Eriksson S FEBS J. 2007 Dec;274(24):6403-14. Epub 2007 Nov 15. PMID:18021254^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang L. The role of Ureaplasma nucleoside monophosphate kinases in the synthesis of nucleoside triphosphates. FEBS J. 2007 Apr;274(8):1983-90. PMID:17355283 doi:10.1111/j.1742-4658.2007.05742.x
↑ Egeblad-Welin L, Welin M, Wang L, Eriksson S. Structural and functional investigations of Ureaplasma parvum UMP kinase--a potential antibacterial drug target. FEBS J. 2007 Dec;274(24):6403-14. Epub 2007 Nov 15. PMID:18021254 doi:http://dx.doi.org/10.1111/j.1742-4658.2007.06157.x
↑ Egeblad-Welin L, Welin M, Wang L, Eriksson S. Structural and functional investigations of Ureaplasma parvum UMP kinase--a potential antibacterial drug target. FEBS J. 2007 Dec;274(24):6403-14. Epub 2007 Nov 15. PMID:18021254 doi:http://dx.doi.org/10.1111/j.1742-4658.2007.06157.x

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2va1"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF UMP KINASE FROM UREAPLASMA PARVUM==
-<StructureSection load='2va1' size='340' side='right' caption='[[2va1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+==Crystal structure of UMP kinase from Ureaplasma parvum==
+<StructureSection load='2va1' size='340' side='right'caption='[[2va1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2va1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27815 Atcc 27815]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VA1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2va1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ureaplasma_parvum Ureaplasma parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VA1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2va1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2va1 OCA], [http://pdbe.org/2va1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2va1 RCSB], [http://www.ebi.ac.uk/pdbsum/2va1 PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2va1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2va1 OCA], [https://pdbe.org/2va1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2va1 RCSB], [https://www.ebi.ac.uk/pdbsum/2va1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2va1 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PYRH_UREPA PYRH_UREPA] Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor. Is also able to phosphorylate dUMP.<ref>PMID:17355283</ref> <ref>PMID:18021254</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2va1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2va1_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 32: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 27815]]
+[[Category: Large Structures]]
-[[Category: Egeblad-Welin, L]]
+[[Category: Ureaplasma parvum]]
-[[Category: Eriksson, S]]
+[[Category: Egeblad-Welin L]]
-[[Category: Wang, L]]
+[[Category: Eriksson S]]
-[[Category: Welin, M]]
+[[Category: Wang L]]
-[[Category: Amino acid kinase family]]
+[[Category: Welin M]]
-[[Category: Kinase]]
-[[Category: Pyrimidine biosynthesis]]
-[[Category: Transferase]]
-[[Category: Umpk]]
-[[Category: Uridylate kinase]]

2va1

From Proteopedia

Current revision

Crystal structure of UMP kinase from Ureaplasma parvum

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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