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2vb2
From Proteopedia
(Difference between revisions)
(New page: 200px<br /><applet load="2vb2" size="350" color="white" frame="true" align="right" spinBox="true" caption="2vb2, resolution 1.70Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:2vb2.jpg|left|200px]]<br /><applet load="2vb2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2vb2, resolution 1.70Å" /> | ||
| - | '''CRYSTAL STRUCTURE OF CU(I)CUSF'''<br /> | ||
| - | == | + | ==Crystal structure of Cu(I)CusF== |
| - | Methionine-rich motifs have an important role in copper trafficking | + | <StructureSection load='2vb2' size='340' side='right'caption='[[2vb2]], [[Resolution|resolution]] 1.70Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2vb2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VB2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vb2 OCA], [https://pdbe.org/2vb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vb2 RCSB], [https://www.ebi.ac.uk/pdbsum/2vb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vb2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CUSF_ECOLI CUSF_ECOLI] Part of a cation efflux system that mediates resistance to copper and silver. Binds one copper per polypeptide.<ref>PMID:11399769</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vb/2vb2_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vb2 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Methionine-rich motifs have an important role in copper trafficking factors, including the CusF protein. Here we show that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies demonstrate that both thioether ligation and strong cation-pi interactions with tryptophan stabilize metal binding. This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry. | ||
| - | + | Cu(I) recognition via cation-pi and methionine interactions in CusF.,Xue Y, Davis AV, Balakrishnan G, Stasser JP, Staehlin BM, Focia P, Spiro TG, Penner-Hahn JE, O'Halloran TV Nat Chem Biol. 2008 Feb;4(2):107-9. Epub 2007 Dec 23. PMID:18157124<ref>PMID:18157124</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2vb2" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: Balakrishnan | + | <references/> |
| - | [[Category: Davis | + | __TOC__ |
| - | [[Category: Focia | + | </StructureSection> |
| - | [[Category: Halloran | + | [[Category: Large Structures]] |
| - | [[Category: Penner-Hahn | + | [[Category: Balakrishnan G]] |
| - | [[Category: Spiro | + | [[Category: Davis AV]] |
| - | [[Category: Staehlin | + | [[Category: Focia P]] |
| - | [[Category: Stasser | + | [[Category: O'Halloran TV]] |
| - | [[Category: Xue | + | [[Category: Penner-Hahn JE]] |
| - | + | [[Category: Spiro TG]] | |
| - | + | [[Category: Staehlin BM]] | |
| - | + | [[Category: Stasser JP]] | |
| - | + | [[Category: Xue Y]] | |
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Current revision
Crystal structure of Cu(I)CusF
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Categories: Large Structures | Balakrishnan G | Davis AV | Focia P | O'Halloran TV | Penner-Hahn JE | Spiro TG | Staehlin BM | Stasser JP | Xue Y
