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2waq
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:2waq.jpg|left|200px]] | ||
| - | + | ==The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase== | |
| - | + | <StructureSection load='2waq' size='340' side='right'caption='[[2waq]], [[Resolution|resolution]] 3.35Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2waq]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_shibatae Saccharolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WAQ FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35Å</td></tr> | |
| - | - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2waq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2waq OCA], [https://pdbe.org/2waq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2waq RCSB], [https://www.ebi.ac.uk/pdbsum/2waq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2waq ProSAT]</span></td></tr> | |
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RPO2_SACSH RPO2_SACSH] DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (Probable). This subunit is involved in DNA promoter recognition (PubMed:22848102).<ref>PMID:22848102</ref> <ref>PMID:19419240</ref> <ref>PMID:21265742</ref> <ref>PMID:22848102</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/2waq_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2waq ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 A resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble. | ||
| - | + | Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure.,Korkhin Y, Unligil UM, Littlefield O, Nelson PJ, Stuart DI, Sigler PB, Bell SD, Abrescia NG PLoS Biol. 2009 May 5;7(5):e102. PMID:19419240<ref>PMID:19419240</ref> | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2waq" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| - | + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | == | + | [[Category: Large Structures]] |
| - | + | [[Category: Saccharolobus shibatae]] | |
| - | + | [[Category: Abrescia NGA]] | |
| - | == | + | [[Category: Bell SD]] |
| - | < | + | [[Category: Korkhin Y]] |
| - | [[Category: | + | [[Category: Littlefield O]] |
| - | [[Category: Abrescia | + | [[Category: Nelson PJ]] |
| - | [[Category: Bell | + | [[Category: Sigler PB]] |
| - | [[Category: Korkhin | + | [[Category: Stuart DI]] |
| - | [[Category: Littlefield | + | [[Category: Unligil UM]] |
| - | [[Category: Nelson | + | |
| - | [[Category: Sigler | + | |
| - | [[Category: Stuart | + | |
| - | [[Category: Unligil | + | |
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Current revision
The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase
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