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2wfl

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{{STRUCTURE_2wfl| PDB=2wfl | SCENE= }}
 
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===CRYSTAL STRUCTURE OF POLYNEURIDINE ALDEHYDE ESTERASE===
 
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{{ABSTRACT_PUBMED_19496101}}
 
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==About this Structure==
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==Crystal structure of polyneuridine aldehyde esterase==
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[[2wfl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WFL OCA].
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<StructureSection load='2wfl' size='340' side='right'caption='[[2wfl]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wfl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WFL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wfl OCA], [https://pdbe.org/2wfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wfl RCSB], [https://www.ebi.ac.uk/pdbsum/2wfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wfl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PNAE_RAUSE PNAE_RAUSE] Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.<ref>PMID:10691977</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wf/2wfl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wfl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cutting carbons: The three-dimensional structure of polyneuridine aldehyde esterase (PNAE) gives insight into the enzymatic mechanism of the biosynthesis of C(9)- from C(10)-monoterpenoid indole alkaloids (see scheme). PNAE is a very substrate-specific serine esterase. It harbors the catalytic triad S87-D216-H244, and is a new member of the alpha/beta-fold hydrolase superfamily. Its novel function leads to the diversification of alkaloid structures.
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Structural basis and enzymatic mechanism of the biosynthesis of C9- from C10-monoterpenoid indole alkaloids.,Yang L, Hill M, Wang M, Panjikar S, Stockigt J Angew Chem Int Ed Engl. 2009;48(28):5211-3. PMID:19496101<ref>PMID:19496101</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wfl" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
*[[Polyneuridine Aldehyde Esterase|Polyneuridine Aldehyde Esterase]]
*[[Polyneuridine Aldehyde Esterase|Polyneuridine Aldehyde Esterase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019496101</ref><references group="xtra"/>
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__TOC__
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[[Category: Polyneuridine-aldehyde esterase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rauvolfia serpentina]]
[[Category: Rauvolfia serpentina]]
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[[Category: Hill, M.]]
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[[Category: Hill M]]
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[[Category: Panjikar, S.]]
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[[Category: Panjikar S]]
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[[Category: Stoeckigt, J.]]
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[[Category: Stoeckigt J]]
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[[Category: Wang, M.]]
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[[Category: Wang M]]
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[[Category: Yang, L.]]
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[[Category: Yang L]]
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[[Category: Alkaloid metabolism]]
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[[Category: Hydrolase]]
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[[Category: Monoterpenoid indole alkaloid]]
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[[Category: Pnae]]
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[[Category: Serine esterase]]
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Current revision

Crystal structure of polyneuridine aldehyde esterase

PDB ID 2wfl

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