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2g0l

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[[Image:2g0l.png|left|200px]]
 
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{{STRUCTURE_2g0l| PDB=2g0l | SCENE= }}
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==Solution Structure of Neocarzinostatin Apo-Protein with bound Flavone==
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<StructureSection load='2g0l' size='340' side='right'caption='[[2g0l]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2g0l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G0L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLN:2-PHENYL-4H-CHROMEN-4-ONE'>FLN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g0l OCA], [https://pdbe.org/2g0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g0l RCSB], [https://www.ebi.ac.uk/pdbsum/2g0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g0l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCZS_STRCZ NCZS_STRCZ] NCS has antibiotic activity (for Gram-positive bacteria) and antitumor activity (for certain mouse tumors). NCS binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Neocarzinostatin (NCS) is a 1:1 complex of an enediyne chromophore (NCSChrom), non-covalently bound to an 11 kDa protein (apoNCS). We are exploring apoNCS as a generic protein system for sequestering small molecules for therapeutic applications. Here, we disclose a new flavone ligand 1 for apoNCS and present a high-resolution NMR structure of this ligand bound to apoNCS. This is the first high-resolution structure of a completely non-cognate ligand bound to the apoNCS protein. This work provides unambiguous evidence that a completely new class of ligand can bind specifically to apoNCS. Furthermore, the mode of binding is different than that of the naphthoate-based ligands, and for such a simple hydrophobic compound, the new ligand surprisingly binds specifically. This work indicates that apo-Neocarzinostatin has multiple selective and distinct binding modes for small-molecule cargo.
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===Solution Structure of Neocarzinostatin Apo-Protein with bound Flavone===
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Synthetic ligands for apo-neocarzinostatin.,Caddick S, Muskett FW, Stoneman RG, Woolfson DN J Am Chem Soc. 2006 Apr 5;128(13):4204-5. PMID:16568976<ref>PMID:16568976</ref>
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{{ABSTRACT_PUBMED_16568976}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2g0l" style="background-color:#fffaf0;"></div>
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[[2g0l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_carzinostaticus Streptomyces carzinostaticus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G0L OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:016568976</ref><ref group="xtra">PMID:012269815</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces carzinostaticus]]
[[Category: Streptomyces carzinostaticus]]
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[[Category: Caddick, S.]]
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[[Category: Caddick S]]
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[[Category: Muskett, F W.]]
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[[Category: Muskett FW]]
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[[Category: Stoneman, R G.]]
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[[Category: Stoneman RG]]
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[[Category: Woolfson, D N.]]
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[[Category: Woolfson DN]]
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[[Category: Antibiotic]]
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[[Category: Beta sandwich]]
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[[Category: Igg fold]]
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Current revision

Solution Structure of Neocarzinostatin Apo-Protein with bound Flavone

PDB ID 2g0l

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