This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2jm3

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)

Structural highlights

2jm3 is a 1 chain structure with sequence from Caenorhabditis elegans. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CTBP1_CAEEL Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.^[1] ^[2]

Publication Abstract from PubMed

The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers.

Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP).,Liew CK, Crossley M, Mackay JP, Nicholas HR J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nicholas HR, Lowry JA, Wu T, Crossley M. The Caenorhabditis elegans protein CTBP-1 defines a new group of THAP domain-containing CtBP corepressors. J Mol Biol. 2008 Jan 4;375(1):1-11. Epub 2007 Oct 22. PMID:18005989 doi:http://dx.doi.org/10.1016/j.jmb.2007.10.041
↑ Chen S, Whetstine JR, Ghosh S, Hanover JA, Gali RR, Grosu P, Shi Y. The conserved NAD(H)-dependent corepressor CTBP-1 regulates Caenorhabditis elegans life span. Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1496-501. doi:, 10.1073/pnas.0802674106. Epub 2009 Jan 21. PMID:19164523 doi:http://dx.doi.org/10.1073/pnas.0802674106
↑ Liew CK, Crossley M, Mackay JP, Nicholas HR. Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP). J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978 doi:S0022-2836(06)01598-1

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jm3"

@@ Line 1: / Line 1: @@
 ==Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)==
-<StructureSection load='2jm3' size='340' side='right' caption='[[2jm3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2jm3' size='340' side='right'caption='[[2jm3]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jm3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JM3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JM3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jm3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JM3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">F49E10.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jm3 OCA], [http://pdbe.org/2jm3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jm3 RCSB], [http://www.ebi.ac.uk/pdbsum/2jm3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jm3 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jm3 OCA], [https://pdbe.org/2jm3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jm3 RCSB], [https://www.ebi.ac.uk/pdbsum/2jm3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jm3 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CTBP1_CAEEL CTBP1_CAEEL]] Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.<ref>PMID:18005989</ref> <ref>PMID:19164523</ref>
+[https://www.uniprot.org/uniprot/CTBP1_CAEEL CTBP1_CAEEL] Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.<ref>PMID:18005989</ref> <ref>PMID:19164523</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Caeel]]
+[[Category: Caenorhabditis elegans]]
-[[Category: Crossley, M]]
+[[Category: Large Structures]]
-[[Category: Liew, C K]]
+[[Category: Crossley M]]
-[[Category: Mackay, J P]]
+[[Category: Liew CK]]
-[[Category: Nicholas, H R]]
+[[Category: Mackay JP]]
-[[Category: Metal binding protein]]
+[[Category: Nicholas HR]]
-[[Category: Protein]]
-[[Category: Zinc finger]]

2jm3

From Proteopedia

Current revision

Solution structure of the THAP domain from C. elegans C-terminal binding protein (CtBP)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox