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2jmh
From Proteopedia
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| - | [[Image:2jmh.jpg|left|200px]] | ||
| - | + | ==NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis== | |
| - | + | <StructureSection load='2jmh' size='340' side='right'caption='[[2jmh]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2jmh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JMH FirstGlance]. <br> | |
| - | | | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jmh OCA], [https://pdbe.org/2jmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jmh RCSB], [https://www.ebi.ac.uk/pdbsum/2jmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jmh ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| - | + | [https://www.uniprot.org/uniprot/ALL5_BLOTA ALL5_BLOTA] | |
| - | + | == Evolutionary Conservation == | |
| - | + | [[Image:Consurf_key_small.gif|200px|right]] | |
| - | == | + | Check<jmol> |
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jmh_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jmh ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases. | Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases. | ||
| - | + | Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen.,Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH Structure. 2008 Jan;16(1):125-36. PMID:18184590<ref>PMID:18184590</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 2jmh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Blomia tropicalis]] | [[Category: Blomia tropicalis]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Chang | + | [[Category: Chang C]] |
| - | [[Category: Chua | + | [[Category: Chua K]] |
| - | [[Category: Huang | + | [[Category: Huang T]] |
| - | [[Category: Kuo | + | [[Category: Kuo I]] |
| - | [[Category: Naik | + | [[Category: Naik MT]] |
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Current revision
NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis
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Categories: Blomia tropicalis | Large Structures | Chang C | Chua K | Huang T | Kuo I | Naik MT
