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2jmh

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[[Image:2jmh.jpg|left|200px]]
 
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==NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis==
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The line below this paragraph, containing "STRUCTURE_2jmh", creates the "Structure Box" on the page.
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<StructureSection load='2jmh' size='340' side='right'caption='[[2jmh]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jmh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JMH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jmh OCA], [https://pdbe.org/2jmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jmh RCSB], [https://www.ebi.ac.uk/pdbsum/2jmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jmh ProSAT]</span></td></tr>
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{{STRUCTURE_2jmh| PDB=2jmh | SCENE= }}
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</table>
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== Function ==
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'''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis'''
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[https://www.uniprot.org/uniprot/ALL5_BLOTA ALL5_BLOTA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jmh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jmh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases.
Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases.
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==About this Structure==
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Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen.,Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH Structure. 2008 Jan;16(1):125-36. PMID:18184590<ref>PMID:18184590</ref>
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2JMH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMH OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18184590 18184590]
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</div>
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<div class="pdbe-citations 2jmh" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Blomia tropicalis]]
[[Category: Blomia tropicalis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chang, C.]]
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[[Category: Chang C]]
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[[Category: Chua, K.]]
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[[Category: Chua K]]
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[[Category: Huang, T.]]
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[[Category: Huang T]]
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[[Category: Kuo, I.]]
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[[Category: Kuo I]]
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[[Category: Naik, M T.]]
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[[Category: Naik MT]]
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[[Category: Allergen]]
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[[Category: Blo t 5]]
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[[Category: Blomia tropicali]]
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[[Category: Dust mite]]
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[[Category: Group 5]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:01:55 2008''
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Current revision

NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis

PDB ID 2jmh

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