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2jnf

From Proteopedia

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[[Image:2jnf.jpg|left|200px]]
 
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{{Structure
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==Solution structure of fly troponin C, isoform F1==
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|PDB= 2jnf |SIZE=350|CAPTION= <scene name='initialview01'>2jnf</scene>
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<StructureSection load='2jnf' size='340' side='right'caption='[[2jnf]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2jnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lethocerus_indicus Lethocerus indicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JNF FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= tnC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=212017 Lethocerus indicus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnf OCA], [https://pdbe.org/2jnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jnf RCSB], [https://www.ebi.ac.uk/pdbsum/2jnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jnf ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q868D4_9HEMI Q868D4_9HEMI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/2jnf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jnf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.
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'''Solution structure of fly troponin C, isoform F1'''
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The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles.,De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A Structure. 2007 Jul;15(7):813-24. PMID:17637342<ref>PMID:17637342</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2jnf" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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To gain a molecular description of how muscles can be activated by mechanical stretch, we have solved the structure of the calcium-loaded F1 isoform of troponin C (TnC) from Lethocerus and characterized its interactions with troponin I (TnI). We show that the presence of only one calcium cation in the fourth EF hand motif is sufficient to induce an open conformation in the C-terminal lobe of F1 TnC, in contrast with what is observed in vertebrate muscle. This lobe interacts in a calcium-independent way both with the N terminus of TnI and, with lower affinity, with a region of TnI equivalent to the switch and inhibitory peptides of vertebrate muscles. Using both synthetic peptides and recombinant proteins, we show that the N lobe of F1 TnC is not engaged in interactions with TnI, excluding a regulatory role of this domain. These findings provide insights into mechanically stimulated muscle contraction.
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*[[Troponin 3D structures|Troponin 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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2JNF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lethocerus_indicus Lethocerus indicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNF OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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The structure of Lethocerus troponin C: insights into the mechanism of stretch activation in muscles., De Nicola G, Burkart C, Qiu F, Agianian B, Labeit S, Martin S, Bullard B, Pastore A, Structure. 2007 Jul;15(7):813-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17637342 17637342]
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[[Category: Lethocerus indicus]]
[[Category: Lethocerus indicus]]
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[[Category: Single protein]]
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[[Category: Bullard B]]
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[[Category: Bullard, B.]]
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[[Category: De Nicola GF]]
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[[Category: Nicola, G F.De.]]
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[[Category: Pastore A]]
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[[Category: Pastore, A.]]
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[[Category: ef-hand]]
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[[Category: lethocerus indicus]]
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[[Category: metal binding protein]]
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[[Category: stretch activated muscle contraction]]
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[[Category: troponin c]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:43:12 2008''
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Current revision

Solution structure of fly troponin C, isoform F1

PDB ID 2jnf

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