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2wok

From Proteopedia

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{{Seed}}
 
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[[Image:2wok.png|left|200px]]
 
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<!--
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==Clavulanic acid biosynthesis oligopeptide binding protein 2 complexed with bradykinin==
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The line below this paragraph, containing "STRUCTURE_2wok", creates the "Structure Box" on the page.
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<StructureSection load='2wok' size='340' side='right'caption='[[2wok]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wok]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WOK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2wok| PDB=2wok | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wok OCA], [https://pdbe.org/2wok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wok RCSB], [https://www.ebi.ac.uk/pdbsum/2wok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wok ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8KRB4_STRCL Q8KRB4_STRCL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wo/2wok_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wok ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Clavulanic acid (CA) is a clinically important beta-lactamase inhibitor that is produced by fermentation of Streptomyces clavuligerus. The CA biosynthesis pathway starts from arginine and glyceraldehyde-3-phosphate and proceeds via (3S,5S)-clavaminic acid, which is converted to (3R,5R)-clavaldehyde, the immediate precursor of (3R,5R)-CA. Open reading frames 7 (orf7) and 15 (orf15) of the CA biosynthesis cluster encode oligopeptide-binding proteins (OppA1 and OppA2), which are essential for CA biosynthesis. OppA1/2 are proposed to be involved in the binding and/or transport of peptides across the S. clavuligerus cell membrane. Peptide binding assays reveal that recombinant OppA1 and OppA2 bind di-/tripeptides containing arginine and certain nonapeptides including bradykinin. Crystal structures of OppA2 in its apo form and in complex with arginine or bradykinin were solved to 1.45, 1.7, and 1.7 A resolution, respectively. The overall fold of OppA2 consists of two lobes with a deep cavity in the center, as observed for other oligopeptide-binding proteins. The large cavity creates a peptide/arginine binding cleft. The crystal structures of OppA2 in complex with arginine or bradykinin reveal that the C-terminal arginine of bradykinin binds similarly to arginine. The results are discussed in terms of the possible roles of OppA1/2 in CA biosynthesis.
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===CLAVULANIC ACID BIOSYNTHESIS OLIGOPEPTIDE BINDING PROTEIN 2 COMPLEXED WITH BRADYKININ===
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Crystal structures of an oligopeptide-binding protein from the biosynthetic pathway of the beta-lactamase inhibitor clavulanic acid.,Mackenzie AK, Valegard K, Iqbal A, Caines ME, Kershaw NJ, Jensen SE, Schofield CJ, Andersson I J Mol Biol. 2010 Feb 19;396(2):332-44. Epub 2009 Nov 24. PMID:19941870<ref>PMID:19941870</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wok" style="background-color:#fffaf0;"></div>
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<!--
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19941870}}, adds the Publication Abstract to the page
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19941870 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_19941870}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Homo sapiens]]
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2WOK is a 2 chains structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WOK OCA].
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[[Category: Large Structures]]
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[[Category: Streptomyces clavuligerus]]
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==Reference==
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[[Category: Andersson I]]
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<ref group="xtra">PMID:19941870</ref><references group="xtra"/>
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[[Category: Caines MEC]]
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[[Category: Andersson, I.]]
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[[Category: Iqbal A]]
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[[Category: Caines, M E.C.]]
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[[Category: Jensen SE]]
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[[Category: Iqbal, A.]]
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[[Category: Kershaw NJ]]
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[[Category: Jensen, S E.]]
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[[Category: Mackenzie AK]]
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[[Category: Kershaw, N J.]]
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[[Category: Schofield CJ]]
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[[Category: Mackenzie, A K.]]
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[[Category: Valegard K]]
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[[Category: Schofield, C J.]]
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[[Category: Valegard, K.]]
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[[Category: Bradykinin]]
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[[Category: Clavulanic acid biosynthesis]]
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[[Category: Oligopeptide]]
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[[Category: Oligopeptide binding protein]]
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[[Category: Peptide binding protein-peptide complex]]
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[[Category: Solute-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 10 16:10:50 2010''
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Current revision

Clavulanic acid biosynthesis oligopeptide binding protein 2 complexed with bradykinin

PDB ID 2wok

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